方案摘要
方案下载应用领域 | 制药/生物制药 |
检测样本 | 其他 |
检测项目 | 其他 |
参考标准 | 暂无 |
Peak-based fraction collection triggered by a UV signal threshold leads to satisfactory results in most cases. Some samples and applications, however, require special attention when fraction collection parameters are set. This Technical Overview describes different strategies on how to achieve best collection results using different settings on an Agilent 1260 Infinity II Preparative LC/MSD System. UV and mass-based fraction collection, fraction pooling, as well as recovery collection are discussed in detail to provide knowledge that can be turned into successful and confident fraction collection.
Abstract
Peak-based fraction collection triggered by a UV signal threshold leads to satisfactory results in most cases. Some samples and applications, however, require special attention when fraction collection parameters are set. This Technical Overview describes different strategies on how to achieve best collection results using different settings on an Agilent 1260 Infinity II Preparative LC/MSD System. UV and mass-based fraction collection, fraction pooling, as well as recovery collection are discussed in detail to provide knowledge that can be turned into successful and confident fraction collection.
Introduction
Agilent InfinityLab LC Purification Solutions1 mark a giant leap in efficient purification. The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector offers the highest flexibility regarding fraction collection modes and configuration of the fraction bed2 . The Agilent 1260 Infinity II Preparative LC/MSD System provides the base for successful fraction collection with a plus in usability.
In a compact housing, the Agilent 1260 Infinity II Preparative Binary Pump delivers high performance for preparative separations using columns from 9.4 up to 30 mm inside diameter. Samples are injected much faster with the Agilent 1260 Infinity II Preparative Autosampler, while carryover has been reduced significantly by a redesigned needle seat and integrated needle wash. With the Agilent extended portfolio of preparative fraction collectors, the system can be tailored to every need. For extra reliability, add an Agilent 1260 Infinity II Preparative Valve-Based Fraction Collector as a recovery collector, diverting the fraction collector waste outlet to distinct recovery positions.
To get the most out of the 1260 Infinity II Preparative LC/MSD System, fraction collection parameters need to be set properly. Although, in most cases, simple peak-based fraction collection triggered by the signal of an ultraviolet (UV) detector will produce satisfactory results, some scenarios might require special attention during method setup. This Technical Overview demonstrates different strategies on how to exploit the vast possibilities of the Agilent OpenLAB CDS ChemStation software to succeed with fraction collection even with challenging samples.
Conclusion
The Agilent 1260 Infinity II Preparative LC/MSD System excels in usability and focus on applications. The broad flexibility of the hardware is complemented by a large number of software features that provide the basis for successful fraction collection under variable conditions. If peak-based fraction collection triggered by a UV signal threshold is not enough to attack a challenging purification scenario, slope triggers, combined with MSD signals, fraction pooling, and recovery collection are only few of the possibilities a 1260 Infinity II Preparative LC/MS System has to offer
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