1290 Infinity II 制备型开放式馏分收集器基于时间、峰、质谱触发的馏分收集

Abstract 

The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector sets new benchmarks in efficient and flexible fraction collection. The fraction bed can be equipped individually with six containers holding fraction tubes in eight different sizes. Three drawers provide easy access to collected fractions even during a run. Peak- and time-based fraction modes have been extended to eight combined modes, enabling the collection of volume or time slices, and including two recovery collection modes. Trigger combinations of ultraviolet (UV) with mass-selective detection (MSD) signals now include an AND/OR logic that gives extra flexibility for challenging purifications. This Technical Overview provides a deeper insight and typical application examples of the fraction modes, including collection results.

Introduction

Fraction collection is the key feature that turns an analytical high-performance liquid chromatography (HPLC) system into a purification system. Simple as it may sound, fraction collection in general, and preparative-scale purification in particular, do have challenging facets. Flow rates in preparative-scale HPLC range from 5 up to 100 mL/min or above, generating fraction volumes of below 1 mL up to tens or even hundreds of mL. This demands collection vessels of different sizes, if the fraction collector capacity is to be used in an optimal way. Quick processing of collected fractions (re-analysis, pooling, and solvent evaporation) is desirable, creating the need for an easily accessible fraction bed. 

The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector1 contains new and improved features that address all of these topics. Fraction containers holding tubes of different diameters (12, 16, 25, 30 mm) and lengths (100 or 150 mm) are optimized to hold fractions of 8 to 78 mL. The fraction bed can be equipped individually with six containers, and holds up to 432 single fractions, or a maximum fraction volume of 5,940 mL. Three drawers separate the fraction bed, and enable the exchange of full drawers even during operation. Only the currently active drawer is locked to prevent spills and sample loss. 

Tubing kits with different inside diameters are available for optimized setup according to the flow rate used. As with the Agilent 1260 Infinity fraction collectors, the 1290 Infinity II Preparative Open-Bed Fraction Collector features a built-in delay sensor, enabling precise calibration of the delay between the detector and the fraction valve without any hardware modifications. 

Along with hardware improvements, the 1290 Infinity II Preparative Open-Bed Fraction Collector offers several enhanced fraction collection modes that combine peak- with time-based collection, and include recovery collections. Fraction collection triggered by a combination of ultraviolet (UV) with mass-selective detection (MSD) signals has also been extended by a third mode, combining the advantages of logical AND/OR connections. This Technical Overview describes the different fraction collection modes available in the 1290 Infinity II Preparative Open-Bed Fraction Collector, and presents typical applications of the feature enhancements.

Conclusion

Besides a larger capacity, and a much more flexible configuration of the fraction bed, the Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector offers new combined fraction modes that are tailored to the demands of different purification challenges. This Technical Overview provides application examples and collection outcomes of these fraction modes, including time-based collection combined with volume slices, peak-based collection combined with time slices, and two recovery collection modes. Peakbased fraction collection is demonstrated with a combination of ultraviolet (UV) and mass-selective detection (MSD) signals, connected by Boolean AND, as well as the novel AND/OR logic