方案摘要
方案下载| 应用领域 | 生物产业 |
| 检测样本 | 生物能源 |
| 检测项目 | |
| 参考标准 | / |
Introduction The term photobleaching refers to loss of fluorescence emission from samples due to prolonged exposure to exciting radiation. While many fluorophores retain stable fluorescence after extended periods of illumination, some photobleach after short periods of time. In order to maintain optimal fluorescence emission, it is important to minimize photobleaching. Strategies to achieve this usually involve limiting one or both of the following: (1) the time of exposure to, or (2) the intensity of, the exciting light. However, either of these strategies may compromise the quality of the results or limit the types of analyses that can be performed because the signal to noise ratio (S/N) is unavoidably decreased. Furthermore, kinetics-based assays performed over an extended period of time may not be possible due to increased exposure of the fluorophore to the excitation light which results in photobleaching.
Blue Fluorescent Protein (BFP) provides an example of a fluorophore that is susceptible to photobleaching: “…even with folding improvements, BFP still suffers from a relatively low fluorescence quantum yield [compared to green fluorescent protein] and relatively easy bleaching.” (Tsien, 1998)1. Despite the useful spectral properties of BFP for use in multi-labelling studies and techniques such as FRET (fluorescence resonance energy transfer),2 it is likely that its low quantum yield and susceptibility to photobleaching could limit ongoing detection in instruments that irradiate the sample continuously, or too intensely, or both. Accordingly, the present study aimed to determine whether using the Agilent Cary Eclipse fluorescence
spectrophotometer, which utilizes a special xenon flash lamp, photobleaching of BFP could be eliminated, or at least reduced, compared to other commercially available instruments having continuous or pulsed light sources.
文献贡献者
使用 Agilent 5800 ICP-OES 测定固态 电解质锂镧锆钽氧 (LLZTO) 中的 主量元素
Agilent Vaya 手持式拉曼光谱仪的 不透明容器分析能力
使用 Agilent 8890 气相色谱和 TCD/FID 系统分析氢气中的氦气、 氩气、氮气和烃类杂质
相关产品
CrossLab企业资产管理服务
Agilent GC 8890 气相色谱系统
Agilent Cary 3500 紫外可见分光光度计
Agilent Resolve 手持拉曼系统
Agilent TRS100 激光拉曼系统
Agilent 1290 Infinity II 二维液相色谱解决方案
Agilent 1290 Infinity II 液相色谱系统
Agilent 1260 Infinity II 液相色谱系统
Agilent 7900 电感耦合等离子体质谱仪
Agilent Bravo 自动液体处理平台
Agilent 7010C 三重四极杆气质联用系统
Agilent 7000E 三重四极杆气质联用系统
Agilent 5977C GC/MSD 单四极杆气质联用仪
Agilent 1290 Infinity II 高通量系统
Agilent 1290 Infinity II 方法转移系统
关注
拨打电话
留言咨询
