Dynamic MRM/Scan Mode: Adding More Confidence to Sensitive Quantitation in Complex Foods by Triple Quadrupole GC/MS (GC/TQ)

2024/02/26   下载量: 0

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应用领域 食品/农产品
检测样本 酱腌菜
检测项目
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Abstract This application note describes the use of the novel simultaneous dynamic multiple reaction monitoring (dMRM) and scan (dMRM/scan) data acquisition mode for triple quadrupole gas chromatography mass spectrometry (GC/TQ) analysis of pesticides in challenging food matrices. The simultaneous dMRM/scan capability enables identification of the unknown compounds and retrospective analysis, while maintaining sensitivity and dynamic range of the method comparable to a conventional dMRM analysis. Additionally, scan data enables more confidence in compound identification by library spectrum matching. Finally, the full scan data allow the analyst to evaluate the sample matrix to ensure the most efficient performance of the GC/TQ system. This work demonstrates the application of dMRM/scan to the analysis of extracts, using Agilent QuEChERS sample preparation, of spinach, walnut, and cayenne pepper spiked with over 200 pesticides. The calibration results and method sensitivity for 203 evaluated compounds were comparable to results observed with conventional dMRM data acquisition mode with the Agilent 8890/7000E GC/TQ and the Agilent 8890/7010C GC/TQ. The unknown identification workflow based on the spectral library matching using a retention time locked library was carried out with Agilent MassHunter Unknowns Analysis. Many of the compounds with the established maximum residue limits (MRLs) were identified with full scan data at concentrations below their MRLs even in the challenging cayenne pepper extract

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Introduction


Concern about trace-level food contaminants is driving the demand for robust, rapid, and reliable methods for identification and quantitation of chemical residues and contaminants in food matrices. Usually, the detection methods such as triple quadrupole GC/MS and triple quadrupole LC/MS are aimed at a specific list of targets that are commonly found in food samples. These methods can be effective but may overlook any residues that are not specifically targeted. The approach to overcome this challenge is to perform untargeted screening of the sample intending to find as many compounds of concern as possible and allowing for retrospective analysis. Untargeted screening can be accomplished by analyzing the sample in full scan data acquisition mode.1,2 However, targeted triple quadrupole GC/MS (GC/TQ) analysis has an advantage of higher sensitivity and selectivity for the target analytes when compared to full scan analysis. The novel simultaneous dynamic MRM and scan (dMRM/scan) allows for acquiring both targeted dMRM GC/TQ data for target quantitation as well as full scan data for unknowns screening. Also, the simultaneous dynamic MRM and scan (dMRM/scan) deliver confident identification based on spectral library matching


上一篇 使用配备氦气节省模块以进行载气 切换的 Agilent 8850 气相色谱系统 分析香草提取物的方法转换
下一篇 使用支持低能量 EI 离子源的新型高 分辨率 GC/Q-TOF 筛查食品基质中 的农药及其他污染物

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