生物大分子分析系统MP-SPR测量药物和蛋白相互作用实验的重复性和再现性

2016-09-13 11:52  下载量:13

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生物大分子分析系统MP-SPR测量药物和蛋白相互作用实验的重复性和再现性: IgG dissociation kinetics from immobilized Protein A was studied using real-time Multi-Parametric Surface Plasmon Resonance (MP-SPR). Various dissociation bu?ers were tested to determine the most e?cient solution. A unique feature, PureKinetics?, allows di?erentiation of real binding from interfering bulk signal artifacts, providing a pure binding signal. The method can clear out even extremely large bulk signals, such as high-ionic strength dissociation bu?ers. In this study, the most e?cient dissociation from Protein A was achieved with bu?ers of pH below 4.0.

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