其他null检测 | 向日葵细胞悬浮培养-维根技术（北京）有限公司（WIGGENS）

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应用领域	农/林/牧/渔
检测样本	其他
检测项目
参考标准	向日葵细胞悬浮培养

The cultivation of Helianthus annuus suspension cultures in single‐use bioreactors of different culture volume should be adapted and established by determining process parameters. A comparison with the cultivation attempts conducted by the TU Dresden (Geipel, Werner) should point out the advantages and disadvantages of the culture systems concerning growth rate and foam generation.

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The cultivation of plant cells continuously became more important in the last years due to a high amount of different substances which could be used by the pharmaceutical and cosmetic industry. However, during cultivation of plant suspension foam generation and shear stress occurs which may have negative effect on cell

growth. In addition, cell growth, aggregation and the release of exopolysaccarides lead to a continuous change of the rheological behaviour, which requires constant process adjustment.

Besides STR’s (stirred tank reactor), disposable (single‐use) systems such as the Biostat CultiBag RM, which is well established in cultivation volumes of 2 to 20 L, are used for plant cell cultivation. By inducing an orbitally shaking motion or a wave‐mixed motion, mixing is achieved and oxygen is transferred from the aerated reactor chamber into the culture medium.

上一篇均质器在饲料中细菌总数的测定实验中的作用

下一篇离心机及其转子的种类，你了解多少？

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维根技术（北京）有限公司（WIGGENS）

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