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Abstract：Pseudostellarin B (cyclic peptide) was isolated and purified from the herbs of Pseudostellaria heterophylla (Miq.) Pax for the first time by high-speed counter-current chromatography (HSCCC) using a two-phase solvent system consisting of n-butanol–ethyl acetate–water (0.6:4.4:5,v/v).The technique can isolate mg levels of the target compound per run with purity better than 96%. The chemical structure of the compound has been positively confirmed by electrospray ionization time of flight (TOF) MS,H-NMR,C-NMR and H-C COSY analyses.
文献贡献者
上海同田生物技术：运用高速逆流色谱法从仙茅中分离仙茅甙和仙茅甙乙
上海同田生物技术：高速逆流色谱法分离制备丹酚酸B
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