方案摘要
方案下载| 应用领域 | 农/林/牧/渔 |
| 检测样本 | 渔业 |
| 检测项目 | 营养成分>粗脂肪, 粗蛋白 |
| 参考标准 | GB∕T 6433-2006饲料粗脂肪测定方法;GB/T 6432-2018饲料中粗蛋白的测定凯氏定氮法 |
使用格哈特公司凯氏定氮仪测定虹鳟鱼鱼肉，及其粪便中蛋白质含量，使用格哈特公司海卓森Hydrotherm全自动超级酸水解结合索克森Soxtherm全自动快速索氏提取仪测定虹鳟鱼鱼肉，及其粪便中总脂肪含量。
Effects of the type of dietary non-protein energy source on the size and composition of the total body bile acid pool, on faecal bile acid loss and on bile acid synthesis were investigated in rainbow trout. Two diets were formulated (similar DP:DE ratio) that differed in the inclusion of either maize starch (Starch) or rapeseed oil (Fat) as main non-protein source. Fish were fed to satiation for 44 days. Type of non-protein energy source did not substantially affect the body bile acid pool composition. However, feeding the Starch diet resulted in a larger total body bile acid pool size compared with the Fat diet, and this despite enhanced faecal bile acid loss when feeding the Starch diet that was related to more faeces being produced. Bile acid synthesis in fish fed the Starch diet was more than two times higher compared with fish fed the Fat diet. The difference in body bile acid pool size between diets suggests upregulation of bile acid synthesis in fish fed the Starch diet beyond the level needed to compensate for the higher faecal bile acid loss and/or downregulation of bile acid synthesis in fish fed the Fat diet. The underlying mechanisms for this difference in synthesis need further investigation.
在彩虹鳟鱼中研究了膳食非蛋白质能量来源类型对全身双酸池大小和组成，对粪便双酸损失和双酸合成的影响。制定了两种饮食（类似的DP：DE比率），不同的是包括玉米淀粉（淀粉）或菜籽油（脂肪）作为主要的非蛋白质来源。鱼被喂食了44天。非蛋白质能量来源的类型并没有显著影响身体的双酸池成分。然而，与脂肪饮食相比，喂食淀粉饮食会导致更大的身体总胆酸池大小，尽管当喂食淀粉饮食时，粪便胆酸的损失会增加，这与产生更多的粪便有关。淀粉饲料中的氢氟酸合成量是脂肪饲料中氢氟酸合成量的两倍多。不同饮食之间体内双酸池大小的差异表明，淀粉饮食中鱼类中双酸合成的上调超过了补偿较高粪便双酸损失和/或脂肪饮食中鱼类中双酸合成的下调所需的水平。这种合成差异的根本机制需要进一步研究。
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