供货周期: | 一周 |
品牌: | |
规格: | 100ul |
货号: | 2478-1 |
CAS号: |
AE1 Phospho(pY359)(SLC4A1)antibody
Cat.#: 2478-1
Rabbit Monoclonal Antibody
Clone ID: EPR1427Y
Swiss Prot: P02730
Mol Weight: 100kDa
Size: 100ul
Description
The AE1 (anion exchanger, band 3) protein is expressed in erythrocytes and in the A-type intercalated cells of the kidney distal collecting tubule. In both cell types it mediates the electroneutral transport of chloride and bicarbonate ions across the lipid bilayer, and, in erythrocytes (1). AE1 is phosphorylated on Tyr359 and Tyr404 by Lyn in erythrocytes. Phosphorylation of these residues is necessary for correct localization and recycling of kidney AE1 to the basolateral membrane (2).
Recommended Applications
WB
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
Species Reactivity
Human
Cross reactivity determined by western blot only.
Products Data
A. Western blot analysis on red blood cell lysate using anti-Phospho-AE1 (pY359) RabMAb (cat. #2478-1), 1:50,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.
Specificity
A phospho specific peptide corresponding to residues surrounding tyrosine 359 of human AE1 was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Alternative Names
SLC4A1 antibody, AE1 antibody, BND3 antibody, CD233 antibody, DI antibody, EMPB3 antibody, EPB3 antibody, FR antibody, MGC116750 antibody, MGC116753 antibody, MGC126619 antibody, MGC126623 antibody, RTA1A antibody, SW antibody, WD antibody, WD1 antibody, WR antibody, Band 3 anion transport protein antibody, Solute carrier family 4 member 1 antibody
Description References
1. Sahr KE, et al. Genomics 24(3):491-501, 1994
2. Toye AM et al. Biochem Soc Symp 72:47-63, 2005
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