供货周期: | 一周 |
品牌: | Cusabio |
规格: | 96T |
货号: | CSB-E08336r |
CAS号: | CSB-E08336r |
产品名称: 大鼠P53(P53)ELISA Kit
英文名称: Rat p53/tumor protein,p53/TP53 ELISA Kit
货号: CSB-E08336r
规格: 96T
种属: Rat
待测物名称: tumor protein p53
缩写: TP53
蛋白功能1: Apoptosis/Autophagy
蛋白功能3: Apoptosis
样本类型: serum, plasma, tissue homogenates
检测范围: 12.5 pg/ml-800 pg/ml
灵敏度: 3.12 pg/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
大鼠P53(P53)ELISA Kit
用途: For research use only. Not for diagnostic use.
大鼠P53(P53)ELISA Kit
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TP53 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TP53 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TP53 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TP53 bound in the initial step. The color development is stopped and the intensity of the color is measured.
大鼠P53(P53)ELISA Kit
特异性: This assay has high sensitivity and excellent specificity for detection of Rat TP53. No significant cross-reactivity or interference between Rat TP53 and analogues was observed.
大鼠P53(P53)ELISA Kit
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
大鼠P53(P53)ELISA Kit
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
大鼠P53(P53)ELISA Kit
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
大鼠P53(P53)ELISA Kit
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the TP53 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
大鼠P53(P53)ELISA Kit
产品名称 英文名称 货号 规格
人P53(P53)ELISA Kit Human p53/tumor protein,p53/TP53 ELISA Kit CSB-E08334h 96T
小鼠p53(p53)ELISA Kit Mouse p53/tumor protein,p53/TP53 ELISA Kit CSB-E08335m 96T
Sheep Cellular tumor antigen p53(TP53) ELISA kit Sheep Cellular tumor antigen p53(TP53) ELISA kit CSB-EL024077SH 96T
兔p53(p53)ELISA Kit Rabbit p53/tumor protein,p53/TP53 ELISA Kit CSB-E11152Rb 96T
Pig Cellular tumor antigen p53(TP53) ELISA kit Pig Cellular tumor antigen p53(TP53) ELISA kit CSB-EL024077PI 96T
Monkey Cellular tumor antigen p53(TP53) ELISA kit Monkey Cellular tumor antigen p53(TP53) ELISA kit CSB-EL024077RH 96T
Horse Cellular tumor antigen p53(TP53) ELISA kit Horse Cellular tumor antigen p53(TP53) ELISA kit CSB-EL024077HO 96T
Guinea pig Cellular tumor antigen p53(TP53) ELISA kit Guinea pig Cellular tumor antigen p53(TP53) ELISA kit CSB-EL024077GU 96T
Donkey Cellular tumor antigen p53(TP53) ELISA kit Donkey Cellular tumor antigen p53(TP53) ELISA kit CSB-EL024077DK 96T
Dog Cellular tumor antigen p53(TP53) ELISA kit Dog Cellular tumor antigen p53(TP53) ELISA kit CSB-EL024077DO 96T
Chicken Cellular tumor antigen p53(TP53) ELISA kit Chicken Cellular tumor antigen p53(TP53) ELISA kit CSB-EL024077CH 96T
Cat Cellular tumor antigen p53(TP53) ELISA kit Cat Cellular tumor antigen p53(TP53) ELISA kit CSB-EL024077CA 96T
Bovine Cellular tumor antigen p53(TP53) ELISA kit Bovine Cellular tumor antigen p53(TP53) ELISA kit CSB-EL024077BO 96T
抗体
产品名称 货号 规格
Rabbit anti-human tumor protein p53 polyclonal Antibody CSB-PA024077GA01HU 150ul
蛋白
产品名称 货号 规格 蛋白来源
Recombinant Ictalurus punctatus Cellular tumor antigen p53(tp53) 1mg
Recombinant Xiphophorus helleri Cellular tumor antigen p53(tp53) 1mg
Recombinant Marmota monax Cellular tumor antigen p53(TP53) 1mg
Recombinant Platichthys flesus Cellular tumor antigen p53(tp53) 1mg
Recombinant Cricetulus griseus Cellular tumor antigen p53(TP53) 1mg
Recombinant Xenopus laevis Cellular tumor antigen p53(tp53) 1mg
Recombinant Macaca fuscata fuscata Cellular tumor antigen p53(TP53) 1mg
Recombinant Macaca fascicularis Cellular tumor antigen p53(TP53) 1mg
Recombinant Oncorhynchus mykiss Cellular tumor antigen p53(tp53) 1mg
Recombinant Chlorocebus aethiops Cellular tumor antigen p53(TP53) 1mg
Recombinant Rat Cellular tumor antigen p53(Tp53) 1mg
Recombinant Danio rerio Cellular tumor antigen p53(tp53) 1mg
Recombinant Oryzias latipes Cellular tumor antigen p53(tp53) 1mg
Recombinant Bos indicus Cellular tumor antigen p53(TP53) 1mg
Recombinant Mouse Cellular tumor antigen p53(Tp53) 1mg
Recombinant Human Cellular tumor antigen p53(TP53) 1mg
Recombinant Macaca mulatta (Rhesus macaque) Cellular tumor antigen p53(TP53) 1mg
Recombinant Sheep Cellular tumor antigen p53(TP53) 1mg
Recombinant Cat Cellular tumor antigen p53(TP53) 1mg
Recombinant Chicken Cellular tumor antigen p53(TP53) 1mg
Recombinant Bovine Cellular tumor antigen p53(TP53) 1mg
Recombinant Horse Cellular tumor antigen p53(TP53) 1mg
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