人P53(P53)ELISA Kit

 产品名称： 人P53(P53)ELISA Kit
 
 英文名称： Human p53/tumor protein,p53/TP53 ELISA Kit
 
 别名： FLJ92943, LFS1, TRP53, p53, p53 antigen
 
 货号： CSB-E08334h
 
 规格： 96T
 
 种属： Human
 
 待测物名称： tumor protein p53
 
 缩写： TP53
 
 蛋白功能1： Apoptosis/Autophagy
 
 蛋白功能3： Apoptosis
 
 样本类型： serum, cell culture supernates, urine, cerebrospinal fluid (CSF), tissue homogenates, cell lysates
 
 检测范围： 9.38 pg/ml-600 pg/ml
 
 灵敏度： 2.34 pg/ml
 
 反应时间： 1-5h
 
 所需样本体积： 50-100ul
 
 检测波长： 450 nm
 
 用途： For research use only. Not for diagnostic use.
 
 原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TP53 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TP53 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TP53 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TP53 bound in the initial step. The color development is stopped and the intensity of the color is measured. 
 特异性： This assay has high sensitivity and excellent specificity for detection of Human TP53. No significant cross-reactivity or interference between Human TP53 and analogues was observed. 
 精密度： Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 
 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
 
 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
 
 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the TP53 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
 
 相关产品：
 
产品名称 英文名称 货号 规格
 小鼠p53(p53)ELISA Kit  Mouse p53/tumor protein,p53/TP53 ELISA Kit  CSB-E08335m  96T 
        
 Sheep Cellular tumor antigen p53(TP53) ELISA kit  Sheep Cellular tumor antigen p53(TP53) ELISA kit  CSB-EL024077SH  96T 
        
 大鼠P53(P53)ELISA Kit  Rat p53/tumor protein,p53/TP53 ELISA Kit  CSB-E08336r  96T 
        
 兔p53(p53)ELISA Kit  Rabbit p53/tumor protein,p53/TP53 ELISA Kit  CSB-E11152Rb  96T 
        
 Pig Cellular tumor antigen p53(TP53) ELISA kit  Pig Cellular tumor antigen p53(TP53) ELISA kit  CSB-EL024077PI  96T 
        
 Monkey Cellular tumor antigen p53(TP53) ELISA kit  Monkey Cellular tumor antigen p53(TP53) ELISA kit  CSB-EL024077RH  96T 
        
 Horse Cellular tumor antigen p53(TP53) ELISA kit  Horse Cellular tumor antigen p53(TP53) ELISA kit  CSB-EL024077HO  96T 
        
 Guinea pig Cellular tumor antigen p53(TP53) ELISA kit  Guinea pig Cellular tumor antigen p53(TP53) ELISA kit  CSB-EL024077GU  96T 
        
 Donkey Cellular tumor antigen p53(TP53) ELISA kit  Donkey Cellular tumor antigen p53(TP53) ELISA kit  CSB-EL024077DK  96T 
        
 Dog Cellular tumor antigen p53(TP53) ELISA kit  Dog Cellular tumor antigen p53(TP53) ELISA kit  CSB-EL024077DO  96T 
        
 Chicken Cellular tumor antigen p53(TP53) ELISA kit  Chicken Cellular tumor antigen p53(TP53) ELISA kit  CSB-EL024077CH  96T 
        
 Cat Cellular tumor antigen p53(TP53) ELISA kit  Cat Cellular tumor antigen p53(TP53) ELISA kit  CSB-EL024077CA  96T 
        
 Bovine Cellular tumor antigen p53(TP53) ELISA kit  Bovine Cellular tumor antigen p53(TP53) ELISA kit  CSB-EL024077BO  96T 
        
抗体 
产品名称 货号 规格
 Rabbit anti-human tumor protein p53 polyclonal Antibody  CSB-PA024077GA01HU  150ul 
      
蛋白 
产品名称 货号 规格 蛋白来源
 Recombinant Ictalurus punctatus Cellular tumor antigen p53(tp53)  CSB-EP024077ILX  1mg  E.coli 
        
 Recombinant Ictalurus punctatus Cellular tumor antigen p53(tp53)  CSB-YP024077ILX  1mg  Yeast 
        
 Recombinant Xiphophorus helleri Cellular tumor antigen p53(tp53)  CSB-EP024077XBJ  1mg  E.coli 
        
 Recombinant Xiphophorus helleri Cellular tumor antigen p53(tp53)  CSB-YP024077XBJ  1mg  Yeast 
        
 Recombinant Marmota monax Cellular tumor antigen p53(TP53)  CSB-EP024077MQG  1mg  E.coli 
        
 Recombinant Marmota monax Cellular tumor antigen p53(TP53)  CSB-YP024077MQG  1mg  Yeast 
        
 Recombinant Platichthys flesus Cellular tumor antigen p53(tp53)  CSB-EP024077EWX  1mg  E.coli 
        
 Recombinant Platichthys flesus Cellular tumor antigen p53(tp53)  CSB-YP024077EWX  1mg  Yeast 
        
 Recombinant Cricetulus griseus Cellular tumor antigen p53(TP53)  CSB-EP024077DXU  1mg  E.coli 
        
 Recombinant Cricetulus griseus Cellular tumor antigen p53(TP53)  CSB-YP024077DXU  1mg  Yeast 
        
 Recombinant Xenopus laevis Cellular tumor antigen p53(tp53)  CSB-EP024077XBE  1mg  E.coli 
        
 Recombinant Xenopus laevis Cellular tumor antigen p53(tp53)  CSB-YP024077XBE  1mg  Yeast 
        
 Recombinant Macaca fuscata fuscata Cellular tumor antigen p53(TP53)  CSB-EP024077MMX  1mg  E.coli 
        
 Recombinant Macaca fuscata fuscata Cellular tumor antigen p53(TP53)  CSB-YP024077MMX  1mg  Yeast 
        
 Recombinant Macaca fascicularis Cellular tumor antigen p53(TP53)  CSB-EP024077MOV  1mg  E.coli 
        
 Recombinant Macaca fascicularis Cellular tumor antigen p53(TP53)  CSB-YP024077MOV  1mg  Yeast 
        
 Recombinant Oncorhynchus mykiss Cellular tumor antigen p53(tp53)  CSB-EP024077OEI  1mg  E.coli 
        
 Recombinant Oncorhynchus mykiss Cellular tumor antigen p53(tp53)  CSB-YP024077OEI  1mg  Yeast 
        
 Recombinant Chlorocebus aethiops Cellular tumor antigen p53(TP53)  CSB-EP024077DSU  1mg  E.coli 
        
 Recombinant Chlorocebus aethiops Cellular tumor antigen p53(TP53)  CSB-YP024077DSU  1mg  Yeast 
        
 Recombinant Rat Cellular tumor antigen p53(Tp53)  CSB-EP024077RA  1mg  E.coli 
        
 Recombinant Rat Cellular tumor antigen p53(Tp53)  CSB-YP024077RA  1mg  Yeast 
        
 Recombinant Danio rerio Cellular tumor antigen p53(tp53)  CSB-EP024077DIL  1mg  E.coli 
        
 Recombinant Danio rerio Cellular tumor antigen p53(tp53)  CSB-YP024077DIL  1mg  Yeast 
        
 Recombinant Oryzias latipes Cellular tumor antigen p53(tp53)  CSB-EP024077OFH  1mg  E.coli 
        
 Recombinant Oryzias latipes Cellular tumor antigen p53(tp53)  CSB-YP024077OFH  1mg  Yeast 
        
 Recombinant Bos indicus Cellular tumor antigen p53(TP53)  CSB-EP024077BUL  1mg  E.coli 
        
 Recombinant Bos indicus Cellular tumor antigen p53(TP53)  CSB-YP024077BUL  1mg  Yeast 
        
 Recombinant Mouse Cellular tumor antigen p53(Tp53)  CSB-EP024077MO  1mg  E.coli 
        
 Recombinant Mouse Cellular tumor antigen p53(Tp53)  CSB-YP024077MO  1mg  Yeast 
        
 Recombinant Human Cellular tumor antigen p53(TP53)  CSB-EP024077HU  1mg  E.coli 
        
 Recombinant Human Cellular tumor antigen p53(TP53)  CSB-YP024077HU  1mg  Yeast 
        
 Recombinant Macaca mulatta (Rhesus macaque) Cellular tumor antigen p53(TP53)  CSB-EP024077  1mg  E.coli 
        
 Recombinant Macaca mulatta (Rhesus macaque) Cellular tumor antigen p53(TP53)  CSB-YP024077  1mg  Yeast 
        
 Recombinant Sheep Cellular tumor antigen p53(TP53)  CSB-EP024077SH  1mg  E.coli 
        
 Recombinant Sheep Cellular tumor antigen p53(TP53)  CSB-YP024077SH  1mg  Yeast 
        
 Recombinant Cat Cellular tumor antigen p53(TP53)  CSB-EP024077CA  1mg  E.coli 
        
 Recombinant Cat Cellular tumor antigen p53(TP53)  CSB-YP024077CA  1mg  Yeast 
        
 Recombinant Chicken Cellular tumor antigen p53(TP53)  CSB-EP024077CH  1mg  E.coli 
        
 Recombinant Chicken Cellular tumor antigen p53(TP53)  CSB-YP024077CH  1mg  Yeast 
        
 Recombinant Bovine Cellular tumor antigen p53(TP53)  CSB-EP024077BO  1mg  E.coli 
        
 Recombinant Bovine Cellular tumor antigen p53(TP53)  CSB-YP024077BO  1mg  Yeast 
        
 Recombinant Horse Cellular tumor antigen p53(TP53)  CSB-EP024077HO  1mg  E.coli 
        
 Recombinant Horse Cellular tumor antigen p53(TP53)  CSB-YP024077HO  1mg  Yeast