Recombinant Human b-NGF(重组人β神经生长因子)|5μg/20μg/1mg|CYT-579-上海希美化学有限公司

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b NGF Human
Beta Nerve Growth Factor Human Recombinant
Catalog No. CYT-579
Synonyms:
Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.

Introduction:
NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.

Description:
Nerve Growth Factor-beta Human Recombinant produced in E.Coli is a non-covalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical 121 amino acids with a molecular weight of two 13.6 kDa polypeptide monomers.
The NGF-b is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The beta-NGF protein was lyophilized from a 0.2µm filtered solution containing no additives or preservatives.

Solubility:
It is recommended to reconstitute the lyophilized NGF-b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NGF-Beta should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
MSSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA

Biological Activity:
The ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.

Protein content:
UV spectroscopy at 280 nm using the absorbency value of 1.1945 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Recombinant Human b-NGF(重组人β神经生长因子)由上海希美化学有限公司为您提供，货号CYT-579，规格：5μg/20μg/1mg，CAS号：，如您想了解更多关于Recombinant Human b-NGF(重组人β神经生长因子)价格、Recombinant Human b-NGF(重组人β神经生长因子)结构式、批发、用途等信息，欢迎咨询。除供应Recombinant Human b-NGF(重组人β神经生长因子)外，还可为您提供Recombinant Human b-NGF CHO(重组人β神经生长因子CHO)、Recombinant Mouse b-NGF(重组小鼠β神经生长因子)等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。

供货周期：	一周
品牌：	Prospect Biosystems
规格：	5μg/20μg/1mg
货号：	CYT-579
CAS号：