Recombinant Human b-NGF CHO(重组人β神经生长因子CHO)

Recombinant Human b-NGF CHO(重组人β神经生长因子CHO)

参考价:¥800
供货周期: 一周
品牌: Prospect Biosystems
规格: 5μg/20μg/1mg
货号: CYT-246
CAS号:
上海希美化学有限公司
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b NGF Human, CHO
Beta-Nerve Growth Factor Human Recombinant, CHO
Catalog No. CYT-246 
Synonyms:
Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.

Introduction:
NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.


Description:
Nerve Growth Factor-beta Human Recombinant produced in CHO is a homodimer, glycosylated, polypeptide chain containing 2 identical 120 amino acids and having a molecular mass of 16,950 Dalton.
The NGF-b is purified by proprietary chromatographic techniques.


Source:
Chinese Hamster Ovary Cells




Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein was lyophilized from a 0.2µm filtered solution in 30mM sodium acetate pH=5.5 containing 1% HSA.


Solubility:
It is recommended to reconstitute the lyophilized NGF-b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Nerve Growth Factor b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Nerve Growth Factor-beta should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.


Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.


Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ser-Ser-His-Pro.


Biological Activity:
The ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.

Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.1945 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Nerve Growth Factor-b Recombinant as a Reference Standard.


Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Recombinant Human b-NGF CHO(重组人β神经生长因子CHO)由上海希美化学有限公司为您提供,货号CYT-246,规格:5μg/20μg/1mg,CAS号:,如您想了解更多关于Recombinant Human b-NGF CHO(重组人β神经生长因子CHO)价格、Recombinant Human b-NGF CHO(重组人β神经生长因子CHO)结构式、批发、用途等信息,欢迎咨询。除供应Recombinant Human b-NGF CHO(重组人β神经生长因子CHO)外,还可为您提供Recombinant Human b-NGF(重组人β神经生长因子)、Recombinant Mouse b-NGF(重组小鼠β神经生长因子)等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,上海希美化学客户服务电话,售前、售后均可联系。
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