重组人白介素-2 Yeast(Human IL-2 Yeast)

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IL 2 Human, Yeast
Interleukin-2 Human Recombinant, Yeast
Catalog No. CYT-604 
Synonyms:
Interleukin-2, T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Introduction:
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

Description:
Interleukin-2 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15 kDa.
The IL-2 is purified by proprietary chromatographic techniques.

Source:
Saccharomyces cerevisiae

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein (1.1mg/ml) was lyophilized after extensive dialysis against 20mM phosphate buffer to a pH=7.6.

Solubility:
It is recommended to reconstitute the lyophilized Interleukin-2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino Acid Sequence:
A P T S S S T K K T Q L Q L E H L L L D
L Q M I L N G I N N Y K N P K L T R M L
T F K F Y M P K K A T E L K H L Q C L E
E E L K P L E E V L N L A Q S K N F H L
R P R D L I S N I N V I V L E L K G S E
T T F M C E Y A D E T A T I V E F L N R
W I T F C Q S I I S T L T

Biological Activity:
The biological activity is tested in a cell proliferation assay using an IL-2 dependent murine cytotoxic T cell line.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. 
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