重组大鼠白介素-10(Rat IL-10)

重组大鼠白介素-10(Rat IL-10)

参考价:面议
供货周期: 一周
品牌: Prospect Biosystems
规格: 2μg/10μg/1mg
货号: CYT-465
CAS号:
上海希美化学有限公司
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IL 10 Rat
Interleukin-10 Rat Recombinant
Catalog No. CYT-465
Synonyms:
B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.

Introduction:
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.


Description:
IL-10 Recombinant Rat produced in E.coli is a single, glycosylated polypeptide chain containing 160 amino acids and having a molecular mass of 18.7 kDa.
The Interleukin-10 Mouse is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein was lyophilized containing 25mM sodium phosphate buffer pH-7.25 and 100mM NaCl.

Solubility:
It is recommended to reconstitute the lyophilized IL-10 Rat Recombinant in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized IL-10 Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Interleukin10 Rat recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Lys-Gly-His.

Biological Activity:
The ED50 as determined by the dose dependent inhibition of MC/9 proliferation is less than 10 ng/ml.

Protein content:
Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.62 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-10 as a Reference Standard.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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» IL 10 Antibody
» IL 10 Antibody
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重组大鼠白介素-10(Rat IL-10)由上海希美化学有限公司为您提供,货号CYT-465,规格:2μg/10μg/1mg,CAS号:,如您想了解更多关于重组大鼠白介素-10(Rat IL-10)价格、重组大鼠白介素-10(Rat IL-10)结构式、批发、用途等信息,欢迎咨询。除供应重组大鼠白介素-10(Rat IL-10)外,还可为您提供重组人白介素-10 CHO(Human IL-10 CHO)、重组人白介素-10(Human IL-10)等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,上海希美化学客户服务电话,售前、售后均可联系。
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