重组小鼠白介素-13(Mouse IL-13)|2μg/10μg/1mg|CYT-375-上海希美化学有限公司

产品详情

IL 13 Mouse
Interleukin-13 Mouse Recombinant
Catalog No. CYT-375
Synonyms:
Interleukin-13, NC300, ALRH, BHR1, P600, IL-13, IL13.

Introduction:
IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.

Description:
Interleukin-13 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 110 amino acids and having a molecular mass of 12 kDa.
The IL-13 is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein (1mg/ml) was lyophilized with no additives.

Solubility:
It is recommended to reconstitute the lyophilized Interleukin 13 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL13 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95% as determined by SDS-PAGE.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and found to be Met-Pro-Val-Pro-Arg.

Biological Activity:
The ED50 range=2-6 ng/ml, determined by the dose dependent prolifiration of TF-1 cells.

Protein content:
Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-13 as a Reference Standard.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

» IL 13 Human
» IL 13 Human, His
» IL 13 Rat

重组小鼠白介素-13(Mouse IL-13)由上海希美化学有限公司为您提供，货号CYT-375，规格：2μg/10μg/1mg，CAS号：，如您想了解更多关于重组小鼠白介素-13(Mouse IL-13)价格、重组小鼠白介素-13(Mouse IL-13)结构式、批发、用途等信息，欢迎咨询。除供应重组小鼠白介素-13(Mouse IL-13)外，还可为您提供重组人白介素-13（Human IL-13）、重组人白介素-13 His（Human IL-13 His)等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。

供货周期：	一周
品牌：	Prospect Biosystems
规格：	2μg/10μg/1mg
货号：	CYT-375
CAS号：