化药新药研发其他>方法开发检测 | 通过Biotage Isolera快速纯化液相制备色谱帮助进行对传递siRNA的多功能的阳离子脂质的研究by耐士科技-上海鑫欣生物科技有限公司

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应用领域	化药/中药/生物制药
检测样本	化药新药研发
检测项目	其他>方法开发
参考标准	GB/T 8****

Exploitation of the RNA interference (RNAi) pathway offers the promise of new and effective therapies for a wide variety of diseases. Clinical development of new drugs based on this platform technology is still limited, however, by a lack of safe and efficient delivery systems. Here we report the development of a class of structurally versatile cationic lipopolyamines designed specifically for delivery of siRNA which show high levels of target transcript knockdown in a range of cell types in vitro. A primary benefit of these lipids is the ease with which they may be covalently modified by the addition of functional molecules. For in vivo applications one of the core lipids (Staramine) was modified with methoxypolyethylene glycols (mPEGs) of varying lengths. Upon systemic administration, PEGylated Staramine nanoparticles containing siRNA targeting the caveolin-1 (Cav-1) transcript caused a reduction of the Cav-1 transcript of up to 60%, depending on the mPEG length, specifically in lung tissue after 48 h compared to treatment with non-silencing siRNA.In addition, modification with mPEG reduced toxicity associated with intravenous administration. The ability to produce a high level of target gene knockdown in the lung with minimal toxicity demonstrates the potential of these lipopolyamines for use in developing RNAi the rapeutics for pulmonary disease.

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