Dereplication-guided isolation of depsides thielavins S–T and lecanorins D–F from the endophytic fungus Setophoma sp. through Biotage Isolera耐士科技

2015/02/13   下载量： 2 次

方案摘要

方案下载
应用领域
检测样本
检测项目
参考标准

Dereplication methodology using UHPLC–DAD–QTOFMS was applied during the metabolic profiling investigation of the endophyte Setophoma sp., a fungus isolated from symptomless guava fruits. The approach performed allowed a fast analysis of the microbial secondary metabolites. From this fungus, seven highly C-alkylated depsides were isolated and identified as polyketides thielavins S, T, U and V and lecanorins D, E and F. Their structures were elucidated through spectroscopic methods including NMR, HRMS and especially with assistance of HRMS/MS experiments. The compounds were tested for quorum sensing regulation activity in the virulence gene expression of Staphylococcus aureus, but no inhibitory effect was detected. Nevertheless, moderate antibacterial activity was encountered in three of tested depsides, particularly with thielavin T, whose MIC was 6.25 lg/mL against S. aureus.

方案下载
上一篇 耐士科技：使用Biotage Isolera快速纯化液相制备色谱从库拉索芦荟叶皮提取天然的磷酸二酯酶抑制剂by耐士科技
下一篇 Design, synthesis and anti-tuberculosis activity of 1-adamantyl-3-heteroaryl ureas with improved in vitro pharmacokinetic properties through Biotage Isolera耐士科技

文献贡献者

上海鑫欣生物科技有限公司
银牌会员 丨 第14年
查看全部资料
相关仪器 更多
相关方案
更多

相关产品

当前位置： 上海鑫欣生物 方案 Dereplication-guided isolation of depsides thielavins S–T and lecanorins D–F from the endophytic fungus Setophoma sp. through Biotage Isolera耐士科技

关注

拨打电话

留言咨询