供货周期: | 一周 |
品牌: | Cusabio |
规格: | 96T |
货号: | CSB-EL001275MO |
CAS号: |
产品类型:ELISA Kit
产品名称:Mouse Disintegrin and metalloproteinase domain-containing protein 12(ADAM12) ELISA kit
英文名称:Mouse Disintegrin and metalloproteinase domain-containing protein 12(ADAM12) ELISA kit
别名: RP11-295J3.5, MCMP, MCMPMltna, MLTN, MLTNA, a disintegrin and metalloproteinase domain 12 (meltrin alpha)|meltrin alpha
种属: Mouse
待测物名称: ADAM metallopeptidase domain 12
缩写: ADAM12
蛋白功能1: Cell Adhesion
蛋白功能3: Cell adhesion
检测范围: 0.156 ng/ml-10 ng/ml
灵敏度: 0.039 ng/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
实验原理
用纯化的抗体包被微孔板,制成固相载体,往包被抗解整合素样金属蛋白酶12(ADAM12)抗体的微孔中依次加入标本或标准品、生物素化的解整合素样金属蛋白酶12(ADAM12)抗体、HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的解整合素样金属蛋白酶12(ADAM12)呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样品浓度。
试剂盒组成及试剂配制
1. 酶联板(Assay plate ):一块(96孔)。
2. 标准品(Standard):2瓶(冻干品)。
3. 样品稀释液(Sample Diluent):1×20ml/瓶。
4. 生物素标记抗体稀释液(Biotin-antibody Diluent):1×10ml/瓶。
5. 辣根过氧化物酶标记亲和素稀释液 (HRP-avidin Diluent):1×10ml/瓶。
6. 生物素标记抗体(Biotin-antibody):1×120μl/瓶(1:100)
7. 辣根过氧化物酶标记亲和素(HRP-avidin):1×120μl/瓶(1:100)
8. 底物溶液(TMB Substrate):1×10ml/瓶。
9. 浓洗涤液(Wash Buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍。
10. 终止液(Stop Solution):1×10ml/瓶(2N H2SO4)。
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