null检测 | 从寄生曲霉菌丝中萃取RNA-上海漾盟仪器有限公司

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Geno AN SP014 (GG RNA Cryoblock Aspergillus parasiticus mycellium) 从寄生曲霉菌丝中萃取RNA RNA Extraction from Aspergillus parasiticus mycelium With kind permission of J.R. Wilkinson and R. Shivaji, Department of Biochemistry and Molecular Biology, Mississippi State University, P.O. Box 9650, Mississippi State, MS 39762, USA Introduction Recent genomic efforts on toxigenic and nontoxigenic Aspergillus species have advanced our understanding of the biology and genetics of these filamentous fungi. However, it is clear that these complex experiments suffer greatly from the variability in the quality of RNA between each replicate and it is critical to establish a platform to isolate high quality RNA for use in both microarray and qRT-PCR. In that context we describe here RNA isolation of A. parasiticus during a simple carbohydrate shif Conclusion Though several environmental factors can affect the final quality of RNA, which directly affects both microarray and real time experiments, we demonstrate here a highly reliable methodology capable of isolating high quality RNA from small quantities of fungal mycelium. This protocol allows simultaneous processing of large numbers of samples, thus demonstrating the suitability of the Geno/Grinder for high throughput processing of fungal mycelium.

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