从动物组织样品中以高通量机械破碎的方法萃取脂肪酸

2011/11/01   下载量: 1

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Geno AN SP001 (GG Cell Lysis fatty acid extraction from animal tissue) 从动物组织样品中以高通量机械破碎的方法萃取脂肪酸 Mechanical Disruption for High-Throughput Fatty Acid Extraction from Animal Tissue Samples With kind permission from Sanofi-Aventis Germany GmbH The mechanical homogenization for extraction of fatty acids is traditionally performed with an Ultra-Turrax in a buffer solution. However, both the purification and the slow sample preparation process in the analytical laboratory are labour-intensive. This method is no longer suitable for high sample throughput. Therefore it makes sense to combine the increased sample throughput with mechanical cell disruption. The use of 96-well micro titer plates and deep well plates is suitable for this process. The research in biotechnology/ gene technology also often demands the handling of a considerable number of samples. Sample preparation is often the bottleneck in the process of efficient analysis work. Up to now efforts to mechanically disrupt cells through grinding have been based on the modification of traditional ball or swing mills with an adapter for micro titer plates. The Geno/Grinder® 2000 is currently the first available mill specifically designed for cell disruption. The Geno/Grinder is a purpose-built ball mill that was originally conceived for the disruption of plant seeds but can also be used for animal and human samples.

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