null检测 | 古代骨和牙齿中提取DNA-上海漾盟仪器有限公司

方案摘要

方案下载

应用领域
检测样本
检测项目
参考标准

This method is designed to maximize recovery of PCR-amplifiable DNA from ancient bone and teeth specimens and at the same time to minimize co-extraction of substances that inhibit PCR. This is achieved by a combination of DNA extraction from bone powder using a buffer consisting solely of EDTA and proteinase K, and purification of the DNA by binding to silica in the presence of high concentrations of guanidinium thiocyanate. All steps are performed at room temperature (20–23 1C), thereby reducing further degradation of the already damaged and fragile ancient DNA and providing an optimal trade-off between DNA release and degradation. Furthermore, the purification step removes most of the various types of PCR inhibitors present in ancient bone samples, thereby optimizing the amount of ancient DNA available for subsequent enzymatic manipulation, such as PCR amplification. The protocol presented here allows DNA extraction from ancient bone and teeth with a minimum of working steps and equipment and yields DNA extracts within 2 working days.

方案下载

上一篇 Preparation of ultra®ne TiC±Ni cermet powders

下一篇用SPEX冷冻研磨机研磨人类牙齿而后用悬浮液进样石墨炉原子吸收法测试铜、铅、锰

文献贡献者

上海漾盟仪器有限公司

银牌会员丨第9年

查看全部资料