从寄生曲霉菌丝中萃取DNA

2011/11/03   下载量: 1

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Introduction Recent genomic efforts on toxigenic and nontoxigenic Aspergillus species have advanced our understanding of the biology and genetics of these filamentous fungi. However, it is clear that these complex experiments suffer greatly from the variability in the quality of RNA between each replicate and it is critical to establish a platform to isolate high quality RNA for use in both microarray and qRT-PCR. In that context we describe here RNA isolation of A. parasiticus during a simple carbohydrate shift.

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