null检测 | 从甜菜叶中萃取核酸-上海漾盟仪器有限公司

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应用领域
检测样本
检测项目
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Within the framework of a TILLING project (Targeting Induced Local Lesions In Genomes) nucleic acids from leaf material are to be isolated from potential mutants. First a representative range of EMS (Ethyl methane sulfonate)-mutants is produced from 2000-5000 M2 plants and identified through TILLING. TILLING technology is a new and very effective method of reverse genetics for the production and identification of loss/gain of function-mutations of commercially valuable genes without genetic modification. To establish a TILLING platform with automated DNA extraction and PCR amplification of target genes, nucleic acid extraction of consistent yield and good quality is necessary. The effective detection of point mutation occurs via hetero duplex formation between DNA molecules of wild type and mutant.

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上一篇 Preparation of ultra®ne TiC±Ni cermet powders

下一篇拟南芥中维他命B1诱导的启动依赖于H2O2和NPR1基因–

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上海漾盟仪器有限公司

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