供货周期: | 现货 |
品牌: | |
型号: | 100ug |
货号: | EB09884 |
产品名称:Goat anti-Cryopirin / NALP3 / NLRP3
产品价格:2340
产 地:Everest Biotech
产品货号:EB09884
产品规格:100ug
产品说明:
Target Protein
Principal Names: AGTAVPRL antibody, AII antibody, AII/AVP antibody, AII/AVP receptor-like antibody, AVP antibody, C1orf7 antibody, CIAS1 antibody, CLR1.1 antibody, FCAS antibody, FCU antibody, FLJ95925 antibody, MWS antibody, NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3 antibody, NACHT, LRR and PYD contain antibody, NALP3 antibody, NLR family, pyrin domain containing 3 antibody, NLRP3 antibody
Official Symbol: NLRP3
Accession Number(s): NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1
Human GeneID(s): 114548
Important Comments: This antibody is expected to recognize all reported isoforms (NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1). Reported variants NP_001073289.1 and NP_004886.3 represent identical protein.
Immunogen
Peptide with sequence RDLYEKAKRDEPK-C, from the N-Terminus (near) of the protein sequence according to NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 36kDa band in Human Peripheral Blood Mononucleocytes lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the smallest calculated size of 106kDa according to NP_899632.1. The 36kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported
Species Reactivity
Tested:
Expected from sequence similarity: Human, Dog, Cow
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