真核翻译起始因子2C2抗体|0.2ml/200μg|-上海纪宁实业有限公司

产品详情

在免疫化学技术中影响真核翻译起始因子2C2抗体效率的因素主要有5个方面：①抗体对抗原的亲合性；②抗体对抗原的特异性；③抗原表位结构在该技术中是如何被改变的；④抗体与抗原结合的易接近性；⑤二级试剂的质量和类型

英文名称 Anti-eIF2C2/Ago2

中文名称真核翻译起始因子2C2抗体

别名 Ago 2; AGO2_HUMAN; Argonaute 2; Argonaute2; dAgo2; eIF 2C 2; eIF-2C 2; eIF2C 2; Eif2c2; Eukaryotic translation initiation factor 2C 2; hAgo2; MGC3183; PAZ Piwi domain protein; PPD; Protein argonaute-2; Protein slicer; Q10; Slicer protein.

真核翻译起始因子2C2抗体浓度 1mg/1ml

规格 0.2ml/200μg

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep

产品类型一抗

研究领域细胞生物染色质和核信号表观遗传学

蛋白分子量 predicted molecular weight: 97kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human eIF2C2/Ago2

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5).

Function : Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

Subunit : Interacts with DICER1 through its Piwi domain and with TARBP2 during assembly of the RNA-induced silencing complex (RISC). Together, DICER1, EIF2C2/AGO2 and TARBP2 constitute the trimeric RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC). Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Note however that the term RISC has also been used to describe the trimeric RLC/miRLC. The formation of RISC complexes containing siRNAs rather than miRNAs appears to occur independently of DICER1. Interacts with EIF2C1/AGO1. Also interacts with DDB1, DDX5, DDX6, DDX20, DHX30, DHX36, DDX47, DHX9, EIF6, ELAVL, FXR1, GEMIN4, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, P4HA1, P4HB, RBM4, SART3, TNRC6A, TNRC6B, UPF1 and YBX1. Interacts with the P-body components DCP1A and XRN1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with RBM4; the interaction is modulated under stress-induced conditions, occurs under both cell proliferation and differentiation conditions and in a RNA-and phosphorylation-independent manner. Interacts with LIMD1, WTIP and AJUBA. Interacts with TRIM71.

Subcellular Location : Cytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.

Post-translational modifications : Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity.

Similarity : Belongs to the argonaute family. Ago subfamily.

Contains 1 PAZ domain.

Contains 1 Piwi domain.

Database links : UniProtKB/Swiss-Prot: Q9UKV8.3

半个多世纪以来，抗体一直作为常用的研究工具，因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作，由于抗体的稳定性好，使用并不困难。抗体较其他蛋白不易变性，易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。

选择合适的抗体对免疫化学技术的成功极为重要。没有任何一种来源的抗体能够满足本书所述的各种方法的要求。为了研究某一重要的抗原，应选用具有不同特性的多种抗体。在没有一套良好特性的抗体可用于研究机体中每一种蛋白之前，选择及制备适合各种技术要求的抗体非常必要。

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J00621 人胰岛素样生长因子结合蛋白2(IGFBP2)ELISA试剂盒 Human insulin-like growth factors binding protein 2,IGFBP-2 ELISA Kit 96T/48T

J00622 人胰岛素样生长因子结合蛋白1(IGFBP-1)ELISA试剂盒 Human insulin-like growth factors binding protein 1,IGFBP-1 ELISA Kit 96T/48T

J00623 人粒细胞巨噬细胞集落刺激因子(GM-CSF)ELISA试剂盒 Human Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit 96T/48T

J00624 人胶质细胞系来源的神经营养因子(GDNF)ELISA试剂盒 Human glial cell line-derived neurotrophic factor,GDNF ELISA Kit 96T/48T

J00625 人粒细胞集落刺激因子(G-CSF)ELISA试剂盒 Human Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit 96T/48T

J00626 人碱性成纤维细胞生长因子6(bFGF-6)ELISA试剂盒 Human basic fibroblast growth factor 6,bFGF-6 ELISA Kit 96T/48T

J00627 人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒 Human basic fibroblast growth factor 4,bFGF-4 ELISA Kit 96T/48T

J00628 人酸性成纤维细胞生长因子1(aFGF-1)ELISA试剂盒 Human acidic fibroblast growth factor 1,aFGF-1 ELISA Kit 96T/48T

J00629 人凋亡相关因子配体(FASL)ELISA试剂盒 Human Factor-related Apoptosis ligand,FASL ELISA Kit 96T/48T

J00630 人凋亡相关因子(FAS/CD95)ELISA试剂盒 Human Factor-related Apoptosis,FAS ELISA Kit 96T/48T

J00631 人嗜酸粒细胞趋化蛋白Eotaxin 1(Eotaxin 1/CCL11)ELISA试剂盒 Human Eotaxin 1 ELISA Kit 96T/48T

J00632 人内分泌腺来源的血管内皮生长因子(EG-VEGF)ELISA试剂盒 Human Endocrine gland vascular endothelial growth factor,EG-VEGF ELISA Kit 96T/48T

J00633 人睫状神经营养因子(CNTF)ELISA试剂盒 Human Ciliary Neurotrophic Factor,CNTF ELISA Kit 96T/48T

J00634 人CXC趋化因子受体1(CXCR1)ELISA试剂盒 Human CXC-chemokine receptor 1,CXCR1 ELISA Kit 96T/48T

J00635 人XC趋化因子受体1(XCR1)ELISA试剂盒 Human XC-chemokine receptor 1,XCR1 ELISA Kit 96T/48T

J00636 人二级淋巴组织趋化因子(SLC/CCL21)ELISA试剂盒 Human secondary lymphoid-tissue chemokine,SLC ELISA Kit 96T/48T

J00637 人E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)ELISA试剂盒 Human E-Cadherin,E-Cad ELISA Kit 96T/48T

J00638 人脑源性神经营养因子(BDNF)ELISA试剂盒 Human Brain derived neurotrophic facor,BDNF ELISA Kit 96T/48T

J00639 人神经调节蛋白1(NRG-1)ELISA试剂盒 human Neuregulin 1,NRG-1 ELISA Kit 96T/48T

J00640 人多巴胺D2受体(D2R)ELISA试剂盒 Human dopamine D2 receptor,D2R ELISA Kit 96T/48T

J00641 人神经元凋亡抑制蛋白(NAIP)ELISA试剂盒 Human neuronal apoptosis inhibitory protein,NAIP ELISA Kit 96T/48T

J00642 人食欲素/阿立新B(OX-B)ELISA试剂盒 Human Orexin B,OX-B ELISA Kit 96T/48T

J00643 人促睡眠肽(DSIP)ELISA试剂盒 Human delta sleep-inducing peptide,DSIP ELISA Kit 96T/48T

J00644 人精氨酸加压素(AVP)ELISA试剂盒 Human arginine vasopressin,AVP ELISA Kit 96T/48T

J00645 人垂体腺苷酸环化酶激活肽(PACAP)ELISA试剂盒 Human pituitary adenylate cyclase activating polypeptide,PACAP ELISA Kit 96T/48T

J00646 人微管相关蛋白2(MAP-2)ELISA试剂盒 Human microtubule-associated protein 2,MAP-2 ELISA Kit 96T/48T

J00647 人神经丝蛋白(NF)ELISA试剂盒 Human neurofilament protein,NF ELISA Kit 96T/48T

J00648 人利钾尿肽(KP)ELISA试剂盒 Human kaliuretic peptide,KP ELISA Kit 96T/48T

J00649 人神经降压素(NT)ELISA试剂盒 Human Neurotensin,NT ELISA Kit 96T/48T

J00650 人γ肽(Pγ)ELISA试剂盒 Human Peptide γ,Pγ ELISA Kit 96T/48T

J00651 人C型钠尿肽(CNP)ELISA试剂盒 Human C -type natriuretic peptide,CNP ELISA Kit 96T/48T

J00652 人阿立新A(Orexin A)ELISA试剂盒 Human Orexin A ELISA Kit 96T/48T

J00653 人脑肠肽(BGP/Gehrelin)ELISA试剂盒 Human brain-gut peptides,BGP/Gehrelin ELISA Kit 96T/48T

J00654 人前心钠肽(Pro-ANP)ELISA试剂盒 Human Pro Atrial Natriuretic Peptide,Pro-ANP ELISA Kit 96T/48T

J00655 人制瘤素M受体(OSMR)ELISA试剂盒 human oncostatin M receptor,OSMR ELISA Kit 96T/48T

J00656 人癌蛋白诱导转录物3(OIT3)ELISA试剂盒 human oncoprotein induced transcript 3,OIT3 ELISA Kit 96T/48T

J00657 人P糖蛋白/渗透性糖蛋白(P-gp)ELISA试剂盒 Human permeability glycoprotein,P-gp ELISA Kit 96T/48T

J00658 人大肠癌专一抗原3(CCSA-3)ELISA试剂盒 Human colon cancer-specific antigen-3,CCSA-3 ELISA Kit 96T/48T

J00659 人大肠癌专一抗原2(CCSA-2)ELISA试剂盒 Human colon cancer-specific antigen-2,CCSA-2 ELISA Kit 96T/48T

J00660 人大肠癌专一抗原4(CCSA-4)ELISA试剂盒 Human colon cancer-specific antigen-4,CCSA-4 ELISA Kit 96T/48T

J00661 人肠三叶因子(ITF)ELISA试剂盒 Human Intestinal trefoil factor,ITF ELISA Kit 96T/48T

J00662 人激肽释放酶11(KLK 11)ELISA试剂盒 Human Kallikrein 11,KLK 11 ELISA Kit 96T/48T

J00663 人生长调节致癌基因γ/黑素瘤生激因子(GROγ/CXCL3/MGSA)ELISA试剂盒 Human growth-regulated oncogeneγ/melanoma growth stimulating activity,GROγ/MGSA ELISA Kit 96T/48T

J00664 人生长调节致癌基因β/黑素瘤生激因子(GROβ/CXCL2/MGSA)ELISA试剂盒 Human growth-regulated oncogeneβ/melanoma growth stimulating activity,GROβ/MGSA ELISA Kit 96T/48T

J00665 人美丽线虫凋亡基因(CED-3)ELISA试剂盒 Human caenorhabditis elegans death gene,CED-3 ELISA Kit 96T/48T

J00666 人胸腺白血病抗原(TLa)ELISA试剂盒 Human thymus-leukemia antigen,TLa ELISA Kit 96T/48T

J00667 人肿瘤特异性移植抗原(TSTA)ELISA试剂盒 Human tumor specific transplantation antigen,TSTA ELISA Kit 96T/48T

J00668 人足细胞标记蛋白/足盂蛋白(PCX)ELISA试剂盒 Human Podocalyxin,PCX ELISA Kit 96T/48T

J00669 人乳腺癌易感蛋白1(BRCA-1)ELISA试剂盒 Human breast cancer susceptibility protein 1,BRCA-1 ELISA Kit 96T/48T

J00670 人T细胞急性淋巴母细胞白血病相关抗原(TALLA-1/CD231)ELISA试剂盒 Human T-cell acute lymphoblastic leukemia antigen,TALLA-1 ELISA Kit 96T/48T

J00671 人核仁形成区嗜银蛋白(Ag-NORs)ELISA试剂盒真核翻译起始因子2C2抗体Human Argyrophilic nucleolar organizer region proteins,Ag-NORs ELISA Kit 96T/48T

J00672 人硫氧化还原蛋白(Trx)ELISA试剂盒 Human Thioredoxin,Trx ELISA Kit 96T/48T

J00673 人窖蛋白(Cav-1)ELISA试剂盒 Human Caveolin-1,Cav-1 ELISA Kit 96T/48T

J00674 人普通急性淋巴细胞白血病抗原(CALLA)ELISA试剂盒 Human common acute lymphocytic leukaemia antigen,CALLA ELISA Kit 96T/48T

J00675 人黑色素细胞刺激素(MSH)ELISA试剂盒 Human melanocyte stimulating hormone,MSH ELISA Kit 96T/48T

J00676 人表皮角蛋白(EK)ELISA试剂盒 Human epidermal keratin,EK ELISA Kit 96T/48T

J00677 人细胞角蛋白21-1片段(CYFRA21-1)ELISA试剂盒 Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit 96T/48T

更多真核翻译起始因子2C2抗体价格、批发价、品牌、厂家及抗体详细说明书，都可以免费咨询及索取。

供货周期：	一周
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规格：	0.2ml/200μg
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