肌球蛋白轻链1/3抗体保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues.
Function : Regulatory light chain of myosin. Does not bind calcium.
Subunit : Myosin is a hexamer of 2 heavy chains and 4 light chains.
Subcellular Location : Cytoplasmic
Post-translational modifications : Isoform MLC3 is acetylated at position 2 (By similarity).
Similarity : Contains 3 EF-hand domains.
Database links : UniProtKB/Swiss-Prot: P05976.3
英文名称 Anti-MLC3F/MYL1/3
中文名称 肌球蛋白轻链1/3抗体
别 名 Myosin Skeletal Heavy and Light chains; myosin, light polypeptide 1, alkali; skeletal, fast; A1 catalytic; A2 catalytic; myosin light chain 1/3, skeletal muscle isoform; MLC1/MLC3; MLC1F/MLC3F; myosin light chain A1/A2; myosin light chain alkali 1/2; MLC1F; MLC3F; MYL1_HUMAN.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit
产品类型 一抗
研究领域 细胞生物 免疫学
蛋白分子量 predicted molecular weight: 21kDa
肌球蛋白轻链1/3抗体性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human MYL1/3
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:1000-5000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.