尤文氏肉瘤相关EWS蛋白抗体|0.2ml/200μg|-上海纪宁实业有限公司

产品详情

在免疫化学技术中影响尤文氏肉瘤相关EWS蛋白抗体效率的因素主要有5个方面：①抗体对抗原的亲合性；②抗体对抗原的特异性；③抗原表位结构在该技术中是如何被改变的；④抗体与抗原结合的易接近性；⑤二级试剂的质量和类型

英文名称 Anti-EWSR1/EWS

中文名称尤文氏肉瘤相关EWS蛋白抗体

别名 bK984G1.4; bK984G1.4 Ewing sarcoma breakpoint region 1 protein; Ewing sarcoma breakpoint region 1; Ewing sarcoma breakpoint region 1 protein; Ewings sarcoma EWS Fli1 type 1 oncogene; EWS; EWS oncogene; EWS_HUMAN; EWSR 1; Ewsr1; EWSR1 protein; RNA binding protein EWS; RNA-binding protein EWS.

尤文氏肉瘤相关EWS蛋白抗体浓度 1mg/1ml

规格 0.2ml/200μg

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Monkey, Orangutan

产品类型一抗

研究领域肿瘤细胞生物信号转导转录调节因子细胞类型标志物表观遗传学

蛋白分子量 predicted molecular weight: 68kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human EWSR1/EWS

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 EWS is a nuclear RNA-binding protein. As a result of chromosome translocation, the EWS gene is fused to a variety of transcription factors, including ATF-1 in human neoplasias. In the Ewing family of tumors, the N-terminal domain of EWS is fused to the DNA-binding domain of various ETS transcription factors, including Fli-1, Erg, ETV1, E1AF and FEV. The EWS/Fli-1 chimeric protein acts as a more potent transcriptional activator than Fli-1 and can promote cell transformation. Two functional regions have been identified in EWS; an amino-terminal region (domain A), that has little transactivation activity but transforms efficiently when fused to Fli-1, and a distal region (domain B), which shows transactivation activity but transforms less efficiently when fused to Fli-1.

Function : Might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Subunit : Binds POLR2C, SF1, calmodulin and RNA. Interacts with PTK2B/FAK2 and TDRD3. Binds calmodulin in the presence, but not in the absence, of calcium ion.

Subcellular Location : Nucleus. Cytoplasm. Cell membrane. Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.

Tissue Specificity : Ubiquitous.

Post-translational modifications : Phosphorylated; calmodulin-binding inhibits phosphorylation of Ser-266.

Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8.

DISEASE : Defects in EWSR1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=Chromosomal aberrations involving EWSR1 are found in patients with Ewing sarcoma. Translocation t(11;22)(q24;q12) with FLI1; translocation t(7;22)(p22;q12) with ETV1; translocation t(21;22)(q22;q12) with ERG; translocation t(9;22)(q22-31;q11-12) with NR4A3. Translocation t(2;21;22)(q23;q22;q12) that forms a EWSR1-FEV fusion protein with potential oncogenic activity.

Note=A chromosomal aberration involving EWSR1 is associated with desmoplastic small round cell tumor (DSRCT). Translocation t(11;22)(p13;q12) with WT1.

Note=A chromosomal aberration involving EWSR1 is associated with malignant melanoma of soft parts (MMSP). Translocation t(12;22)(q13;q12) with ATF-1. Malignant melanoma of soft parts, also known as soft tissue clear cell sarcoma, is a rare tumor developing in tendons and aponeuroses.

Note=A chromosomal aberration involving EWSR1 is associated with small round cell sarcoma. Translocation t(11;22)(p36.1;q12) with PATZ1.

Defects in EWSR1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving EWSR1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;22)(q13;q12) with ATF1 generates a chimeric EWSR1/ATF1 protein. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.

Note=EFPS arise due to chromosomal translocations in which EWSR1 is fused to a variety of cellular transcription factors. EFPS are very potent transcriptional activators dependent on the EAD and a C-terminal DNA-binding domain contributed by the fusion partner. The spectrum of malignancies associated with EFPS are thought to arise via EFP-induced transcriptional deregulation, with the tumor phenotype specified by the EWSR1 fusion partner and cell type. Transcriptional repression of the transforming growth factor beta type II receptor (TGF beta RII) is an important target of the EWS-FLI1, EWS-ERG, or EWS-ETV1 oncogene.

Similarity : Belongs to the RRM TET family.

Contains 1 IQ domain.

Contains 1 RanBP2-type zinc finger.

Contains 1 RRM (RNA recognition motif) domain.

Database links : UniProtKB/Swiss-Prot: Q01844.1

半个多世纪以来，抗体一直作为常用的研究工具，因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作，由于抗体的稳定性好，使用并不困难。抗体较其他蛋白不易变性，易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。

选择合适的抗体对免疫化学技术的成功极为重要。没有任何一种来源的抗体能够满足本书所述的各种方法的要求。为了研究某一重要的抗原，应选用具有不同特性的多种抗体。在没有一套良好特性的抗体可用于研究机体中每一种蛋白之前，选择及制备适合各种技术要求的抗体非常必要。

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J00910 人瘢痕性天疱疮抗体(CP)ELISA试剂盒 Human cicatricial Pemphigoid,CP ELISA Kit 96T/48T

J00911 人乙型肝炎病毒前S2抗体(HBV preS2Ab)ELISA试剂盒 human hepatitis B virus,HBV preS2Ab ELISA Kit 96T/48T

J00912 人乙型肝炎病毒前S2抗原(HBV preS2Ag)ELISA试剂盒 human hepatitis B virus,HBV preS2Ag ELISA Kit 96T/48T

J00913 人输血传播病毒/辛型肝炎病毒((TTV)ELISA试剂盒 human transfusion transmitted virus,TTV ELISA Kit 96T/48T

J00914 人去氨加压素/1-去氨基-8-右旋-精氨酸加压素(dDAVP)ELISA试剂盒 Human Desmopressin/1-desamino-8-D-arginine vasopressin,dDAVP ELISA Kit 96T/48T

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J00916 人毒蕈碱型乙酰胆碱受体(M-AChR)ELISA试剂盒 Human muscarinic acetylcholine receptor,M-AChR ELISA Kit 96T/48T

J00917 人食欲素受体(OXR)ELISA试剂盒 Human Orexin receptor,OXR ELISA Kit 96T/48T

J00918 人真胰岛素(TI)ELISA试剂盒 Human true insulin,TI ELISA Kit 96T/48T

J00919 人重酒石酸去甲肾上腺素(NE-B)ELISA试剂盒 Human Noradrenaline Bitartas,NE-B ELISA Kit 96T/48T

J00920 人前列环素(PGI)ELISA试剂盒 Human prostacycline,PGI ELISA Kit 96T/48T

J00921 人反三碘甲状腺原氨酸(rT3)ELISA试剂盒 Human ReverseTri-iodothyronine,rT3 ELISA Kit 96T/48T

J00922 人6酮前列腺素F1a(6-keto-PGF1a)ELISA试剂盒 Human 6-keto-prostaglandin F1a,6-K-PGF1a ELISA Kit 96T/48T

J00923 人戊糖素(Pentosidine)ELISA试剂盒 Human Pentosidine ELISA Kit 96T/48T

J00924 人变肾上腺素(MN) ELISA试剂盒 Human metanephrine,MN ELISA Kit 96T/48T

J00925 人多巴胺D1受体(D1R)ELISA试剂盒 Human Dopamine D1 receptor,D1R ELISA Kit 96T/48T

J00926 人肾上腺素能a1A受体(ADRA1A)ELISA试剂盒 Human adrenergic alpha-1A receptor,ADRA1A ELISA Kit 96T/48T

J00927 人早老素2(PS-2)ELISA试剂盒 Human Presenilin 2,PS-2 ELISA Kit 96T/48T

J00928 人异丙肾上腺素(iso-Hyd)ELISA试剂盒 Human Isoprenaline Hydrochloride,iso-Hyd ELISA Kit 96T/48T

J00929 人胰抑制素(Pancreastatin)ELISA试剂盒 Human Pancreastatin ELISA Kit 96T/48T

J00930 人雄激素受体(AR)ELISA试剂盒 Human androgen receptor,AR ELISA Kit 96T/48T

J00931 人松弛肽/松弛素(RLN)ELISA试剂盒 Human Relaxin,RLN ELISA Kit 96T/48T

J00932 人长效甲状腺刺激素(LATS)ELISA试剂盒 Human Long acting thyroid stimulator,LATS ELISA Kit 96T/48T

J00933 人抗利尿激素/血管加压素/精氨酸加压素(ADH/VP/AVP)ELISA试剂盒 Human antidiuretic hormone/vasopressin/arginine vasopressin,ADH/VP/AVP ELISA Kit 96T/48T

J00934 人甘丙肽/甘丙素(GAL)ELISA试剂盒 Human galanin,GAL ELISA Kit 96T/48T

J00935 人促胰液素/分泌素受体(SR)ELISA试剂盒 Human secretin receptor,SR ELISA Kit 96T/48T

J00936 人超敏生长激素(U S-GH)ELISA试剂盒 Human Ultra Sensitive growth hormone,U S-GH ELISA Kit 96T/48T

J00937 人超敏前列腺特异性抗原(PSA-U S)ELISA试剂盒 Human Ultra Sensitive Prostate Specific Antigen,PSA U S ELISA Kit 96T/48T

J00938 人苯丙酸诺龙/苯丙酸去甲睾酮(NP)ELISA试剂盒 Human Nandrolone Phenylpropionate,NP ELISA Kit 96T/48T

J00939 人16α羟基雌酮1(16-α OHE-1)ELISA试剂盒 Human 16-αHydroxyestrogen 1,16-α OHE-1 ELISA Kit 96T/48T

J00940 人甲状旁腺激素相关蛋白(PTHrP)ELISA试剂盒 Human Parathyroid Hormone Related Protein,PTHrP ELISA Kit 96T/48T

J00941 人甲状腺素抗体(TAb)ELISA试剂盒 Human Parathyroid Hormone Related Protein,PTHrP ELISA Kit 96T/48T

J00942 人半胱氨酸蛋白酶抑制剂/胱抑素C(Cys-C)ELISA试剂盒 Human Cystatin C,Cys-C ELISA Kit 96T/48T

J00943 人性激素结合球蛋白(SHBG)ELISA试剂盒 Human sex hormone-binding globulin,SHBG ELISA Kit 96T/48T

J00944 人脱氢表雄酮S7(DHEA-S7)ELISA试剂盒 Human Dehydroepiandrosterone S7,DHEA-S7 ELISA Kit 96T/48T

J00945 人脱氢表雄酮(DHEA)ELISA试剂盒 Human Dehydroepiandrosterone,DHEA ELISA Kit 96T/48T

J00946 人降钙素基因相关肽(CGRP)ELISA试剂盒 Human calcitonin gene related peptide,CGRP ELISA Kit 96T/48T

J00947 人6酮前列腺素(6-K-PG)ELISA试剂盒 Human 6-keto-prostaglandin,6-K-PG ELISA Kit 96T/48T

J00948 人大内皮素(Big ET)ELISA试剂盒 Human Big Endothelin,Big ET ELISA Kit 96T/48T

J00949 人全段甲状旁腺素(i-PTH)ELISA试剂盒 Human intact Parathormone ,i-PTH ELISA Kit 96T/48T

J00950 人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒 Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit 96T/48T

J00951 人新生甲状腺素(NN-T4)ELISA试剂盒 Human Neonatal Thyroxine,NN-T4 ELISA Kit 96T/48T

J00952 人绒毛膜促性腺激素β(β-CG)ELISA试剂盒 Human Chorionic Gonadotrophin β,β-CG ELISA Kit 96T/48T

J00953 人泼尼松龙(PS)ELISA试剂盒 Human Prednisolone,PS ELISA Kit 96T/48T

J00954 人苗条素受体(LR/Ob-R)ELISA试剂盒 Human Leptin receptor,LR/Ob-R ELISA Kit 96T/48T

J00955 人红细胞生成素受体(EPOR)ELISA试剂盒 Human Erythropoietin receptor,EPOR ELISA Kit 96T/48T

J00956 人β-微管蛋白(TUBB)ELISA试剂盒 human β-tubulin,TUBB ELISA Kit 96T/48T

J00957 人总前列腺特异抗原(tPSA)ELISA试剂盒 Human total prostate s pecific antigen,tPSA ELISA Kit 96T/48T

J00958 人解脲脲原体抗体(UU-Ab)ELISA试剂盒 Human Ureaplasma urealyticum antibody,UU-Ab ELISA Kit 96T/48T

J00959 人复合前列腺特异性抗原(CPSA)ELISA试剂盒 Human complex edprostate specific antigen,cPSA ELISA Kit 96T/48T

J00960 人单纯疱疹病毒Ⅱ型抗体(HSVⅡ-Ab)ELISA试剂盒 Human Herpes simplexvirus Ⅱ antibody,HSVⅡ-Ab ELISA Kit 96T/48T

J00961 人细菌性阴道病(BV)ELISA试剂盒 Human bacterial vaginosis,BV ELISA Kit 96T/48T

J00962 人单纯疱疹病毒Ⅰ型抗体(HSVⅠAb)ELISA试剂盒 Human Herpes simplexvirusⅠantibody,HSVⅠAb ELISA Kit 96T/48T

J00963 人雌激素诱导蛋白PS2 ELISA试剂盒 Human Estrogen-induced protein pS2 ELISA Kit 96T/48T

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J00965 人乳头状瘤病毒抗体IgG(HPV-IgG)ELISA试剂盒 human papillomavirus IgG,HPV-IgG ELISA Kit 96T/48T

J00966 人单纯疱疹病毒抗原2(HSV-Ag2)ELISA试剂盒 Human Herpes simplex virus 2,HSV-Ag2 ELISA Kit 96T/48T

J00967 人游离前列腺特异性抗原(fPSA)ELISA试剂盒 Human Free Prostate Specific Antigen,fPSA ELISA Kit 96T/48T

J00968 人前列腺特异性抗原(PSA)ELISA试剂盒 Human prostate specific antigen,PSA ELISA Kit 96T/48T

J00969 人磷脂酰丝氨酸(PS)ELISA试剂盒 Human phosphatidylserine,PS ELISA Kit 96T/48T

更多尤文氏肉瘤相关EWS蛋白抗体价格、批发价、品牌、厂家及抗体详细说明书，都可以免费咨询及索取。

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规格：	0.2ml/200μg
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