英文名称 Anti-phospho-MEK1/MAP2K1(Thr292)
中文名称 磷酸化丝裂原活化蛋白激酶1抗体
别 名 MEK1(Phospho-Thr292); Dual specificity mitogen activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAP/Erk kinase 1; Map2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEKK1; Mitogen activated protein kinase kinase 1; MKK 1; MKK1; PRKMK 1; PRKMK1;Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1); Protein kinase mitogen activated kinase 1; Protein kinase mitogen activated, kinase 1; MP2K1_HUMAN.
磷酸化丝裂原活化蛋白激酶1抗体浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 免疫学 信号转导 转录调节因子 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 43kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human MEK1 around the phosphorylation site of Thr292
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Function : Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Subunit : Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3/ERK1 and RGS14 (By similarity). Forms an heterodimer with MAP2K2/MEK2 (By similarity). Forms heterodimers with KSR2 which further dimerize to form tetramers (By similarity). Interacts with ARBB2, LAMTOR3, MAPK1/ERK2, MORG1 and RAF1 (By similarity). Interacts with PPARG and with isoform 1 of VRK2. Interacts with Yersinia yopJ. Interacts with SGK1. Interacts with BIRC6/bruce.
Subcellular Location : Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole body. Cytoplasm. Nucleus. Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
Tissue Specificity : Widely expressed, with extremely low levels in brain.
Post-translational modifications : Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK.
Similarity : Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Database links : UniProtKB/Swiss-Prot: Q02750.2
丝裂原活化蛋白激酶是一组可以被多种细胞外信号即获得蛋白丝/苏氨酸激酶，处于胞浆信号传导通路的终末位置，活化后转位到核内，作用于核内转录因子，调节基因表达。它主要参与生长因子、激素、细胞因子、应激等各种刺激下细胞的反应、细胞的生长、分化过程。经研究证实，MAPK信号转导通路存在于大多数细胞内，在将细胞外刺激信号转导至细胞及其核内，并引起细胞生物学反应（如细胞增殖、分化、转化及凋亡等）的过程中具有至关重要的作用。研究表明，MAPK信号转导通路在细胞内具有生物进化的高度保守性，在低等原核细胞和高等哺乳类细胞内，目前均已发现存在着多条并行的MAPK信号通路，不同的细胞外刺激可使用不同的MAPK信号通路，通过其相互调控而介导不同的细胞生物学反应。
半个多世纪以来，抗体一直作为常用的研究工具，因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作，由于抗体的稳定性好，使用并不困难。抗体较其他蛋白不易变性，易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。
选择合适的抗体对免疫化学技术的成功极为重要。没有任何一种来源的抗体能够满足本书所述的各种方法的要求。为了研究某一重要的抗原，应选用具有不同特性的多种抗体。在没有一套良好特性的抗体可用于研究机体中每一种蛋白之前，选择及制备适合各种技术要求的抗体非常必要。
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J10682 白细胞激活黏附因子(ALCAM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Activated Leukocyte Cell Adhesion Molecule (ALCAM) 48T/96T Rattus norvegicus (Rat)
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J10687 血管生成素1(ANGPT1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Angiopoietin 1 (ANGPT1) 48T/96T Rattus norvegicus (Rat)
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J10689 血管生成素2(ANGPT2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Angiopoietin 2 (ANGPT2) 48T/96T Mus musculus (Mouse)
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J10695 CD83分子(CD83)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cluster Of Differentiation 83 (CD83) 48T/96T Rattus norvegicus (Rat)
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J10699 内分泌腺来源血管内皮生长因子(EG-VEGF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) 48T/96T Mus musculus (Mouse)
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J10701 红细胞生成素受体(EPOR)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Erythropoietin Receptor (EPOR) 48T/96T Rattus norvegicus (Rat)
J10702 红细胞生成素受体(EPOR)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Erythropoietin Receptor (EPOR) 48T/96T Mus musculus (Mouse)
J10703 E选择素(SELE)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Selectin, Endothelium (SELE) 48T/96T Oryctolagus cuniculus (Rabbit)
J10704 红细胞生成素(EPO)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Erythropoietin (EPO) 48T/96T Rattus norvegicus (Rat)
J10705 凋亡相关因子(FAS)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Factor Related Apoptosis (FAS) 48T/96T Rattus norvegicus (Rat)
J10706 凋亡相关因子配体(FASL)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Factor Related Apoptosis Ligand (FASL) 48T/96T Rattus norvegicus (Rat)
J10707 酸性成纤维细胞生长因子(FGF1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1) 48T/96T Rattus norvegicus (Rat)
J10708 酸性成纤维细胞生长因子(FGF1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1) 48T/96T Mus musculus (Mouse)
J10709 干扰素α(IFNα)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interferon Alpha (IFNa) 48T/96T Sus scrofa; Porcine (Pig)
J10710 干扰素α(IFNα)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interferon Alpha (IFNa) 48T/96T Oryctolagus cuniculus (Rabbit)
J10711 成纤维细胞生长因子6(FGF6)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 6 (FGF6) 48T/96T Rattus norvegicus (Rat)
J10712 成纤维细胞生长因子4(FGF4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 4 (FGF4) 48T/96T Mus musculus (Mouse)
J10713 成纤维细胞生长因子4(FGF4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 4 (FGF4) 48T/96T Rattus norvegicus (Rat)
J10714 成纤维细胞生长因子6(FGF6)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 6 (FGF6) 48T/96T Mus musculus (Mouse)
J10715 成纤维细胞生长因子9(FGF9)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 9 (FGF9) 48T/96T Rattus norvegicus (Rat)
J10716 成纤维细胞生长因子9(FGF9)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 9 (FGF9) 48T/96T Mus musculus (Mouse)
J10717 FMS样酪氨酸激酶3(Flt3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for FMS Like Tyrosine Kinase 3 (Flt3) 48T/96T Rattus norvegicus (Rat)
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J10737 血管内皮生长因子C(VEGFC)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Vascular Endothelial Growth Factor C (VEGFC) 48T/96T Mus musculus (Mouse)
J10738 白介素12B(IL12B)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 12B (IL12B) 48T/96T Rattus norvegicus (Rat)
J10739 血管内皮生长因子B(VEGFB)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Vascular Endothelial Growth Factor B (VEGFB) 48T/96T Rattus norvegicus (Rat)
J10740 白介素12A(IL12A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 12A (IL12A) 48T/96T Rattus norvegicus (Rat)
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J10743 尿激酶型纤溶酶原激活因子受体(uPAR)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plasminogen Activator, Urokinase Receptor (uPAR) 48T/96T Mus musculus (Mouse)
J10744 尿激酶型纤溶酶原激活因子(uPA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plasminogen Activator, Urokinase (uPA) 48T/96T Rattus norvegicus (Rat)
J10745 尿激酶型纤溶酶原激活因子(uPA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plasminogen Activator, Urokinase (uPA) 48T/96T Mus musculus (Mouse)
更多磷酸化丝裂原活化蛋白激酶1抗体价格、批发价、品牌、厂家及抗体详细说明书，都可以免费咨询及索取。
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