英文名称 Anti-phospho-MEK1+MEK2(Ser222/Ser226)
中文名称 磷酸化丝裂原活化蛋白激酶1抗体
别 名 MAP2K1(phospho S222); MAP2K1/MAP2K2(phospho S222); Dual specificity mitogen activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAP/Erk kinase 1; Map2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEKK1; Mitogen activated protein kinase kinase 1; MKK 1; MKK1; PRKMK 1; PRKMK1;Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1); Protein kinase mitogen activated kinase 1; Protein kinase mitogen activated, kinase 1; MP2K1_HUMAN.
磷酸化丝裂原活化蛋白激酶1抗体浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 免疫学 信号转导 转录调节因子 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 43kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human MEK1 around the phosphorylation site of Ser222
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul2008].
Function : Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Subunit : Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3/ERK1 and RGS14 (By similarity). Forms an heterodimer with MAP2K2/MEK2 (By similarity). Forms heterodimers with KSR2 which further dimerize to form tetramers (By similarity). Interacts with ARBB2, LAMTOR3, MAPK1/ERK2, MORG1 and RAF1 (By similarity). Interacts with PPARG and with isoform 1 of VRK2. Interacts with Yersinia yopJ. Interacts with SGK1. Interacts with BIRC6/bruce.
Subcellular Location : Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole body. Cytoplasm. Nucleus. Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
Tissue Specificity : Widely expressed, with extremely low levels in brain.
Post-translational modifications : Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK.
Similarity : Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Database links : UniProtKB/Swiss-Prot: Q02750.2
半个多世纪以来，抗体一直作为常用的研究工具，因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作，由于抗体的稳定性好，使用并不困难。抗体较其他蛋白不易变性，易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。
选择合适的抗体对免疫化学技术的成功极为重要。没有任何一种来源的抗体能够满足本书所述的各种方法的要求。为了研究某一重要的抗原，应选用具有不同特性的多种抗体。在没有一套良好特性的抗体可用于研究机体中每一种蛋白之前，选择及制备适合各种技术要求的抗体非常必要。
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J10814 甲状旁腺激素(PTH)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Parathyroid Hormone (PTH) 48T/96T Rattus norvegicus (Rat)
J10815 甲状旁腺激素(PTH)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Parathyroid Hormone (PTH) 48T/96T Mus musculus (Mouse)
J10816 前列腺素E合酶2(PTGES2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Prostaglandin E Synthase 2 (PTGES2) 48T/96T Mus musculus (Mouse)
J10817 去甲肾上腺素(NE)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Noradrenaline (NE) 48T/96T General
J10818 结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Connective Tissue Growth Factor (CTGF) 48T/96T Homo sapiens (Human)
J10819 结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Connective Tissue Growth Factor (CTGF) 48T/96T Rattus norvegicus (Rat)
J10820 结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Connective Tissue Growth Factor (CTGF) 48T/96T Mus musculus (Mouse)
J10821 维生素D5(VD5)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Vitamin D5 (VD5) 48T/96T Homo sapiens (Human)
J10822 组织因子(TF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Factor (TF) 48T/96T Homo sapiens (Human)
J10823 组织因子(TF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Factor (TF) 48T/96T Rattus norvegicus (Rat)
J10824 组织因子(TF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tissue Factor (TF) 48T/96T Mus musculus (Mouse)
J10825 组织型纤溶酶原激活因子(tPA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plasminogen Activator, Tissue (tPA) 48T/96T Homo sapiens (Human)
J10826 组织型纤溶酶原激活因子(tPA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plasminogen Activator, Tissue (tPA) 48T/96T Rattus norvegicus (Rat)
J10827 组织型纤溶酶原激活因子(tPA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plasminogen Activator, Tissue (tPA) 48T/96T Mus musculus (Mouse)
J10828 免疫球蛋白G3(IgG3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Immunoglobulin G3 (IgG3) 48T/96T Homo sapiens (Human)
J10829 C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for C Reactive Protein (CRP) 48T/96T Cavia (Guinea pig )
J10830 Ⅲ型胶原(COL3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Collagen Type III (COL3) 48T/96T Rattus norvegicus (Rat)
J10831 Ⅲ型胶原(COL3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Collagen Type III (COL3) 48T/96T Mus musculus (Mouse)
J10832 Ⅱ型前胶原氨基端原肽(PⅡNT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Procollagen II N-Terminal Propeptide (PIINP) 48T/96T Homo sapiens (Human)
J10833 Ⅱ型前胶原氨基端原肽(PⅡNT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Procollagen II N-Terminal Propeptide (PIINP) 48T/96T Rattus norvegicus (Rat)
J10834 Ⅱ型前胶原氨基端原肽(PⅡNT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Procollagen II N-Terminal Propeptide (PIINP) 48T/96T Mus musculus (Mouse)
J10835 血小板激活因子(PAF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Platelet Activating Factor (PAF) 48T/96T Homo sapiens (Human)
J10836 血小板激活因子(PAF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Platelet Activating Factor (PAF) 48T/96T Rattus norvegicus (Rat)
J10837 氧化低密度脂蛋白(OxLDL)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL) 48T/96T Homo sapiens (Human)
J10838 血小板衍生生长因子A(PDGFA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Platelet Derived Growth Factor Subunit A (PDGFA) 48T/96T Homo sapiens (Human)
J10839 血小板衍生生长因子A(PDGFA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Platelet Derived Growth Factor Subunit A (PDGFA) 48T/96T Mus musculus (Mouse)
J10840 血小板衍生生长因子A(PDGFA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Platelet Derived Growth Factor Subunit A (PDGFA) 48T/96T Rattus norvegicus (Rat)
J10841 血栓调节蛋白(TM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Thrombomodulin (TM) 48T/96T Homo sapiens (Human)
J10842 血栓调节蛋白(TM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Thrombomodulin (TM) 48T/96T Mus musculus (Mouse)
J10843 血栓调节蛋白(TM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Thrombomodulin (TM) 48T/96T Rattus norvegicus (Rat)
J10844 纤维蛋白原降解产物(FDP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibrinogen Degradation Product (FDP) 48T/96T Homo sapiens (Human)
J10845 纤维蛋白原降解产物(FDP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibrinogen Degradation Product (FDP) 48T/96T Mus musculus (Mouse)
J10846 纤维蛋白原降解产物(FDP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibrinogen Degradation Product (FDP) 48T/96T Rattus norvegicus (Rat)
J10847 纤溶酶原激活物抑制因子2(PAI2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plasminogen Activator Inhibitor 2 (PAI2) 48T/96T Homo sapiens (Human)
J10848 纤溶酶原激活物抑制因子2(PAI2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plasminogen Activator Inhibitor 2 (PAI2) 48T/96T Mus musculus (Mouse)
J10849 纤溶酶原激活物抑制因子2(PAI2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plasminogen Activator Inhibitor 2 (PAI2) 48T/96T Rattus norvegicus (Rat)
J10850 纤溶酶原激活物抑制因子1(PAI1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1) 48T/96T Homo sapiens (Human)
J10851 纤溶酶原激活物抑制因子1(PAI1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1) 48T/96T Mus musculus (Mouse)
J10852 纤溶酶原激活物抑制因子1(PAI1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1) 48T/96T Rattus norvegicus (Rat)
J10853 细胞间粘附分子3(ICAM3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Intercellular Adhesion Molecule 3 (ICAM3) 48T/96T Homo sapiens (Human)
J10854 细胞间粘附分子2(ICAM2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Intercellular Adhesion Molecule 2 (ICAM2) 48T/96T Homo sapiens (Human)
J10855 细胞间粘附分子2(ICAM2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Intercellular Adhesion Molecule 2 (ICAM2) 48T/96T Mus musculus (Mouse)
J10856 细胞间粘附分子2(ICAM2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Intercellular Adhesion Molecule 2 (ICAM2) 48T/96T Rattus norvegicus (Rat)
J10857 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Enolase, Neuron Specific (NSE) 48T/96T Homo sapiens (Human)
J10858 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Enolase, Neuron Specific (NSE) 48T/96T Mus musculus (Mouse)
J10859 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Enolase, Neuron Specific (NSE) 48T/96T Rattus norvegicus (Rat)
J10860 髓鞘碱性蛋白(MBP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Myelin Basic Protein (MBP) 48T/96T Sus scrofa; Porcine (Pig)
J10861 前列腺素E2(PGE2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Prostaglandin E2 (PGE2) 48T/96T General
J10862 脑钠素(BNP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Brain Natriuretic Peptide (BNP) 48T/96T Homo sapiens (Human)
J10863 脑钠素(BNP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Brain Natriuretic Peptide (BNP) 48T/96T Mus musculus (Mouse)
J10864 脑钠素(BNP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Brain Natriuretic Peptide (BNP) 48T/96T Rattus norvegicus (Rat)
J10865 内皮抑素(ES)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Endostatin (ES) 48T/96T Homo sapiens (Human)
J10866 内皮抑素(ES)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Endostatin (ES) 48T/96T Mus musculus (Mouse)
J10867 免疫球蛋白M(IgM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Immunoglobulin M (IgM) 48T/96T Homo sapiens (Human)
J10868 免疫球蛋白M(IgM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Immunoglobulin M (IgM) 48T/96T Mus musculus (Mouse)
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J10870 免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Immunoglobulin G (IgG) 48T/96T Mus musculus (Mouse)
J10871 免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Immunoglobulin G (IgG) 48T/96T Rattus norvegicus (Rat)
J10872 免疫球蛋白E(IgE)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Immunoglobulin E (IgE) 48T/96T Mus musculus (Mouse)
J10873 免疫球蛋白E(IgE)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Immunoglobulin E (IgE) 48T/96T Rattus norvegicus (Rat)
J10874 免疫球蛋白A(IgA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Immunoglobulin A (IgA) 48T/96T Homo sapiens (Human)
J10875 免疫球蛋白A(IgA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Immunoglobulin A (IgA) 48T/96T Mus musculus (Mouse)
J10876 免疫球蛋白A(IgA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Immunoglobulin A (IgA) 48T/96T Rattus norvegicus (Rat)
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J10878 血管细胞粘附分子1(VCAM1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1) 48T/96T Mus musculus (Mouse)
J10879 血管细胞粘附分子1(VCAM1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1) 48T/96T Rattus norvegicus (Rat)
J10880 巨噬细胞炎性蛋白5(MIP5)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Macrophage Inflammatory Protein 5 (MIP5) 48T/96T Homo sapiens (Human)
更多磷酸化丝裂原活化蛋白激酶1抗体价格、批发价、品牌、厂家及抗体详细说明书，都可以免费咨询及索取。
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