磷酸化肌球蛋白轻链抗体保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues.
Subunit : Myosin is an hexamer of 2 heavy chains and 4 light chains.
Tissue Specificity : Smooth muscle tissues and in some, but not all, nonmuscle cells.
Similarity : Contains 3 EF-hand domains.
Database links :
Entrez Gene: 23209 Human
Entrez Gene: 170790 Mouse
Entrez Gene: 315215 Rat
Omim: 605908 Human
SwissProt: Q15049 Human
SwissProt: Q8VHK5 Mouse
Unigene: 517729 Human
Unigene: 32780 Mouse
英文名称 Anti-phospho-MLC(Ser20)/Myosin light chain(Ser20)
中文名称 磷酸化肌球蛋白轻链抗体
别 名 p-MLC(Ser20); phospho-MLC(Ser20); p-Myosin light chain(Ser20); Myosin light chain(Ser20); A1 catalytic; A2 catalytic; Alkali myosin light chain 1; Alkali myosin light chain 3; C36E6.3; C36E6.3; CMH10; DKFZp779C0562; KMLC; MLC 2; MLC 2v; MLC; MLC1F; MLC2; MLC3F; MLCK2; MRLC1; MYL 1; MYL 2; MYL1; MYL1 protein; MYL2; MYL9; MYLK2; Myosin light chain 1 alkali skeletal fast; Myosin light chain 1 skeletal muscle isoform; Myosin light chain 2 regulatory cardiac slow; Myosin light chain 3 skeletal muscle isoform; Myosin light chain kinase 2; Myosin regulatory light chain 2 ventricular/cardiac muscle isoform; Myosin regulatory light chain 2, smooth muscle isoform; Myosin, light chain 9, regulatory; MYRL2; Skeletal myosin light chain kinase.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep
产品类型 一抗 磷酸化抗体
研究领域 心血管 信号转导 干细胞 细胞骨架 细胞膜蛋白
蛋白分子量 predicted molecular weight: 20kDa
磷酸化肌球蛋白轻链抗体性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human Myosin light chain around the phosphorylation site of Ser20
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.