α-L岩藻糖苷酶抗体|0.2ml/200μg |-上海基免实业有限公司

产品详情

α-L岩藻糖苷酶抗体英文名称 Anti-FUCA1/Alpha L fucosidase I

中文名称 α-L岩藻糖苷酶抗体

别名 Alpha L fucosidase I; Alpha L fucoside fucohydrolase; Alpha-L-fucosidase 1; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; FUCA; FUCA1; FUCO_HUMAN; Tissue alpha L fucosidase; Tissue alpha-L-fucosidase.

浓度 1mg/1ml

规格 0.2ml/200μg

纯化的抗体可通过不同的途径获取，有些α-L岩藻糖苷酶抗体可通过下述方法制备或从商家购买。从商家购买的抗体，通常附有正确的储存方法。

1）工作液应在4℃下融化并存放，可能稳定达数月。

2）如果没有特殊原因而避免使用叠氮钠，亦可加入叠氮钠，浓度为0．02％。将纯化的抗体样本分装成合适的体积，于-20℃保存。

3）纯化的抗体溶液应以较高的浓度(如lmg／m1)在中性pH下保存。：常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法)，皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记，可将它们以较低浓度储存于加有1％BSA的溶液中。

4）经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间，即使保存多年，对抗体也无损害。多数情况下，盐浓度适于保持在0-150mmol/L之间，但在长期存放的抗体中，盐溶液浓度高达500mmol/L时，对α-L岩藻糖苷酶抗体可能有损害。如果没有其他说明．律议用PBS或50mmol/LTris(DH8．0)溶液长期存放抗体。

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse

产品类型一抗

研究领域肿瘤细胞生物免疫学信号转导肿瘤细胞生物标志物

蛋白分子量 predicted molecular weight: 50kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human FUCA1/Alpha L fucosidase I

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

α-L岩藻糖苷酶抗体产品介绍 Alpha-L-fucosidase,AFU is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.

Function : Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.

Subunit : Homotetramer.

Subcellular Location : Lysosome.

DISEASE : Fucosidosis (FUCA1D) [MIM:230000]: An autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity : Belongs to the glycosyl hydrolase 29 family.

Database links : UniProtKB/Swiss-Prot: P04066.4

α-L-岩藻糖苷酶是一种催化含岩藻糖基的糖蛋白、糖脂等生物活性大分子水解酶的溶酶体酸性水解酶。其广泛分布于人体组织细胞、血液和体液中。参与体内糖蛋白、糖脂和寡糖的代谢。由于肝癌患者α-L-岩藻糖苷酶明显升高，目前它被认为是原发性肝癌的一种新的肿瘤标记物。α-L岩藻糖苷酶升高：见于原发性肝癌、转移性肝癌、肝硬化、急性肝炎等。

供货周期：	一周
品牌：
规格：	0.2ml/200μg
货号：
CAS号：