4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Horse
产品类型 一抗
研究领域 心血管 细胞生物 免疫学 干细胞 t-淋巴细胞 b-淋巴细胞 细胞因子
蛋白分子量 predicted molecular weight: 23kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human FLT3L (101-150aa)
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
FMS样酪氨酸激酶3配体抗体产品介绍 Flt 3 ligand (Flt 3-L), variously designated Flt 3/Flk 2 ligand or FL, is a hematopoietic growth factor that stimulates the proliferation of stem and CD34+ progenitor cells and has been cloned from both mouse and human genomes. Flt 3-L is a potent in vitro growth stimulator of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and G-CSF-dependent granulocyte-macrophage committed precursors from Lin CD34+ bone marrow cells as well as other primitive B cell populations. Additionally, Flt 3-L stimulates the proliferation of hematopoietic progenitor cells isolated from mouse fetal liver or adult mouse bone marrow. Flt 3-L does not, however, affect the growth of erythroid-committed progenitors. Flt 3-L exists in two forms and is active as both a soluble and as a membrane-bound ligand. The Flt 3-L receptor, Flt 3, is a tyrosine kinase expressed on CD34+ cells that shares a high degree of homology with the SCF (stem cell factor) receptor, c-Kit and c-Fms
Function : Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Subunit : Homodimer (isoform 2).
Subcellular Location : Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
Database links : UniProtKB/Swiss-Prot: P49771.1
Flt3L是一种可以促进多种干细胞、血细胞及其前体细胞生成、分化的细胞因子,对T淋巴细胞、B淋巴细胞和NK细胞和DC的前体细胞都有促进增生、诱导分化的功能.Flt3L可与其它促细胞生长的细胞因子有很好的协同作用.目前,尚未发现具有毒性作用,是一种良好的扩增剂,对研究细胞的形态、细胞表现型和功能具有重要的意义.