胃肠道相关酪氨酸激酶抗体（蛋白酪氨酸激酶5）|0.2ml/200μg |-上海基免实业有限公司

产品详情

胃肠道相关酪氨酸激酶抗体（蛋白酪氨酸激酶5）英文名称 Anti-FRK/PTK5

中文名称胃肠道相关酪氨酸激酶抗体（蛋白酪氨酸激酶5）

别名 Beta cell Src homology tyrosine kinase; BSK; BSK/IYK; C85044; Frk; Frk fyn related kinase; FRK_HUMAN; FYN related kinase; FYN-related kinase; GASK; Gastrointestinal associated kinase; Gastrointestinal associated tyrosine kinase; GTK; Intestine tyrosine kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5; PTK5; RAK; Tyrosine protein kinase FRK; Tyrosine-protein kinase FRK.

浓度 1mg/1ml

规格 0.2ml/200μg

纯化的抗体可通过不同的途径获取，有些胃肠道相关酪氨酸激酶抗体（蛋白酪氨酸激酶5）可通过下述方法制备或从商家购买。从商家购买的抗体，通常附有正确的储存方法。

1）工作液应在4℃下融化并存放，可能稳定达数月。

2）如果没有特殊原因而避免使用叠氮钠，亦可加入叠氮钠，浓度为0．02％。将纯化的抗体样本分装成合适的体积，于-20℃保存。

3）纯化的抗体溶液应以较高的浓度(如lmg／m1)在中性pH下保存。：常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法)，皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记，可将它们以较低浓度储存于加有1％BSA的溶液中。

4）经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间，即使保存多年，对抗体也无损害。多数情况下，盐浓度适于保持在0-150mmol/L之间，但在长期存放的抗体中，盐溶液浓度高达500mmol/L时，对胃肠道相关酪氨酸激酶抗体（蛋白酪氨酸激酶5）可能有损害。如果没有其他说明．律议用PBS或50mmol/LTris(DH8．0)溶液长期存放抗体。

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit

产品类型一抗

研究领域肿瘤细胞生物免疫学细胞周期蛋白激酶和磷酸酶

蛋白分子量 predicted molecular weight: 58kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human FRK/PTK5

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

胃肠道相关酪氨酸激酶抗体（蛋白酪氨酸激酶5）产品介绍 The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq, Jul 2008].

Function : Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.

Subunit : Interacts (via the SH3-domain) with PTEN. Interacts with RB1.

Subcellular Location : Cytoplasm. Nucleus. Note=Predominantly found in the nucleus, with a small fraction found in the cell periphery.

Tissue Specificity : Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon.

Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Database links : UniProtKB/Swiss-Prot: P42685.1

供货周期：	一周
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规格：	0.2ml/200μg
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