4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig
产品类型 一抗
研究领域 肿瘤 细胞生物 生长因子和激素 细胞类型标志物
蛋白分子量 predicted molecular weight: 43kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human FOP/FGFR1OP
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
FGFR1癌基因伴侣蛋白抗体产品介绍 FOP, is a 399 amino acid protein that localizes to the centrosome and contains one LisH domain. Expressed ubiquitously with highest expression in kidney, heart, muscle, colon, liver, testis and pancreas, FOP functions as a homodimer that interacts with EB1 and CEP350 and is essential for anchoring microtubules to centrosomes. Chromosomal aberrations that involve the FOP gene are associated with the pathogenesis of stem cell myeloproliferative disorder (MPD), a condition that is characterized by eosinophilia and myeloid hyperplasia and ultimately leads to acute myeloid leukemia. FOP is expressed as multiple isoforms that are produced by alternative splicing events.
Function : Required for anchoring microtubules to the centrosomes.
Subunit : Homodimer. Part of a ternary complex that contains CEP350, FGFR1OP and MAPRE1. Interacts directly with CEP350 and MAPRE1.
Subcellular Location : Cytoplasm, cytoskeleton, centrosome. Note=Associated with gamma-tubulin.
Tissue Specificity : Ubiquitous. Highly expressed in heart, liver, muscle, kidney, intestine, colon, adrenal gland, prostate, testis, and pancreas.
DISEASE : Note=A chromosomal aberration involving FGFR1OP may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
Similarity : Belongs to the FGFR1OP family.
Contains 1 LisH domain.
Database links : UniProtKB/Swiss-Prot: O95684.1