GATA结合蛋白1伴侣蛋白抗体|0.2ml/200μg |-上海基免实业有限公司

产品详情

GATA结合蛋白1伴侣蛋白抗体英文名称 Anti-FOG1

中文名称 GATA结合蛋白1伴侣蛋白抗体

别名 FOG-1; FOG1_HUMAN; Friend of GATA 1; Friend of GATA protein 1; Friend of GATA1; ZFPM1; Zinc finger protein; Zinc finger protein 89A; Zinc finger protein multitype 1; Zinc finger protein ZFPM1.

浓度 1mg/1ml

规格 0.2ml/200μg

纯化的抗体可通过不同的途径获取，有些GATA结合蛋白1伴侣蛋白抗体可通过下述方法制备或从商家购买。从商家购买的抗体，通常附有正确的储存方法。

1）工作液应在4℃下融化并存放，可能稳定达数月。

2）如果没有特殊原因而避免使用叠氮钠，亦可加入叠氮钠，浓度为0．02％。将纯化的抗体样本分装成合适的体积，于-20℃保存。

3）纯化的抗体溶液应以较高的浓度(如lmg／m1)在中性pH下保存。：常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法)，皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记，可将它们以较低浓度储存于加有1％BSA的溶液中。

4）经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间，即使保存多年，对抗体也无损害。多数情况下，盐浓度适于保持在0-150mmol/L之间，但在长期存放的抗体中，盐溶液浓度高达500mmol/L时，对GATA结合蛋白1伴侣蛋白抗体可能有损害。如果没有其他说明．律议用PBS或50mmol/LTris(DH8．0)溶液长期存放抗体。

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat

产品类型一抗

研究领域心血管细胞生物神经生物学信号转导锌指蛋白表观遗传学

蛋白分子量 predicted molecular weight: 105 kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human FOG1

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

GATA结合蛋白1伴侣蛋白抗体产品介绍 Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2.

Function : Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2 (By similarity).

Subunit : Interacts with corepressor CTBP2; this interaction is however not essential for corepressor activity (By similarity). Interacts with the N-terminal zinc-finger of GATA1, GATA2 and probably GATA3.

Subcellular Location : Nucleus.

Tissue Specificity : Mainly expressed in hematopoietic tissues. Also expressed in adult cerebellum, stomach, lymph node, liver and pancreas. Expressed in fetal heart, liver and spleen.

Similarity : Belongs to the FOG (Friend of GATA) family.

Contains 4 C2H2-type zinc fingers.

Contains 5 C2HC-type zinc fingers.

Database links : UniProtKB/Swiss-Prot: Q8IX07.2

供货周期：	一周
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规格：	0.2ml/200μg
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