成纤维细胞生长因子8抗体|0.2ml/200μg |-上海基免实业有限公司

产品详情

成纤维细胞生长因子8抗体英文名称 Anti-FGF8/HBGF-8

中文名称成纤维细胞生长因子8抗体

别名 AIGF; Androgen induced growth factor; FGF 8; Fibroblast growth factor 8 (androgen induced); Fibroblast growth factor 8 precursor; HBGF 8; HBGF8; FGF8_HUMAN.

浓度 1mg/1ml

规格 0.1ml/100μg 0.2ml/200μg

纯化的抗体可通过不同的途径获取，有些成纤维细胞生长因子8抗体可通过下述方法制备或从商家购买。从商家购买的抗体，通常附有正确的储存方法。

1）工作液应在4℃下融化并存放，可能稳定达数月。

2）如果没有特殊原因而避免使用叠氮钠，亦可加入叠氮钠，浓度为0．02％。将纯化的抗体样本分装成合适的体积，于-20℃保存。

3）纯化的抗体溶液应以较高的浓度(如lmg／m1)在中性pH下保存。：常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法)，皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记，可将它们以较低浓度储存于加有1％BSA的溶液中。

4）经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间，即使保存多年，对抗体也无损害。多数情况下，盐浓度适于保持在0-150mmol/L之间，但在长期存放的抗体中，盐溶液浓度高达500mmol/L时，对成纤维细胞生长因子8抗体可能有损害。如果没有其他说明．律议用PBS或50mmol/LTris(DH8．0)溶液长期存放抗体。

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep

产品类型一抗

研究领域肿瘤细胞生物免疫学

蛋白分子量 predicted molecular weight: 27kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human FGF8 C-terminus

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

成纤维细胞生长因子8抗体产品介绍 Keratinocyte growth factor precursor (KGF) (Fibroblast growth factor 8; FGF-8; HBGF-8). Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells. Cooperates with Wnt-1 in mouse mammary tumor virus-induced murine mammary tumorigenesis. [Subcellular location] Secreted. [Tissue specificity] Absent in normal mammary glands and detected only in adult testis and ovary and in midgestational embryos. [Induction] By androgens. Belongs to the heparin-binding growth factors family.

Function : Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells.

Subunit : Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors.

Subcellular Location : Secreted.

DISEASE : Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6) [MIM:612702]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous.

Similarity : Belongs to the heparin-binding growth factors family.

Database links :

UniProtKB/Swiss-Prot: P55075.1

Entrez Gene: 2253 Human

Omim: 600483 Human

SwissProt: P55075 Human

Unigene: 57710 Human

有研究者认为：纤维母细胞生长因子8 FGF8 对癌症具有一定的抑制作用。

早期研究FGF8，认为是自分泌生长因子，它在前列腺癌、乳癌和卵巢癌中常常表现过量表达 (over-expressed)，并与前列腺癌的格里森分数 (Gleason score) 相关联。经过各种细胞和动物模型的实验均支持FGF8在抑制肿瘤形成和肿瘤细胞成活方面的作用。

此外，还有学者认为：FGF8 还在实验中显示出，他有改善因胶原蛋白引发的关节炎性的改变，如：风湿性关节炎和类风湿性关节炎及退行性骨性关节病。

供货周期：	一周
品牌：
规格：	0.2ml/200μg
货号：
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