促代谢型谷氨酸受体2抗体

促代谢型谷氨酸受体2抗体英文名称  Anti-GRM2/GLUR2 

中文名称  促代谢型谷氨酸受体2抗体 

别    名  Glutamate Receptor Metabotropic 2; Gprc1b; AMPA selective glutamate receptor 2; GLUR 2; GLUR2; GLURB; Glutamate receptor metabotropic 2; Metabotropic Glutamate Receptor 2; GPRC1B; GRM 2; Grm2; mGlu 2; MGlu2; MGLUR 2; Mglur2; GRM2_HUMAN. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg     

纯化的抗体可通过不同的途径获取，有些促代谢型谷氨酸受体2抗体可通过下述方法制备或从商家购买。从商家购买的抗体，通常附有正确的储存方法。

1）工作液应在4℃下融化并存放，可能稳定达数月。

2）如果没有特殊原因而避免使用叠氮钠，亦可加入叠氮钠，浓度为0．02％。将纯化的抗体样本分装成合适的体积，于-20℃保存。

3）纯化的抗体溶液应以较高的浓度(如lmg／m1)在中性pH下保存。：常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法)，皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记，可将它们以较低浓度储存于加有1％BSA的溶液中。

4）经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间，即使保存多年，对抗体也无损害。多数情况下，盐浓度适于保持在0-150mmol/L之间，但在长期存放的抗体中，盐溶液浓度高达500mmol/L时，对促代谢型谷氨酸受体2抗体能有损害。如果没有其他说明．律议用PBS或50mmol/LTris(DH8．0)溶液长期存放抗体。  

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit

产品类型  一抗    

研究领域  肿瘤 细胞生物 免疫学 信号转导 细胞凋亡  

蛋白分子量  predicted molecular weight: 96kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human GRM2/GLUR2 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  ICC=1:100-500  IF=1:50-200 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

促代谢型谷氨酸受体2抗体产品介绍 L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.

Function : Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.

Subunit : Interacts with GRASP (By similarity).

Subcellular Location : Cell membrane; Multi-pass membrane protein. 

Tissue Specificity : Widely expressed in different regions of the adult brain as well as in fetal brain.

Similarity : Belongs to the G-protein coupled receptor 3 family.

Database links : UniProtKB/Swiss-Prot: Q14416.2

谷氨酸受体-代谢能2；主要参与细胞信号转导及突触传递，在G蛋白偶联受体蛋白信号转导途径中有一定的作用，并且有负调控腺苷酸环化酶的作用，为膜性蛋白，在胞膜、胞浆中表达。