供货周期: | 现货 |
品牌: | Abcam |
规格: | 0.2ml/200μg |
货号: | |
CAS号: |
磷脂酰基醇蛋白聚糖-3抗体英文名称 Anti-glypican 3/GPC3
中文名称 磷脂酰基醇蛋白聚糖-3抗体
别 名 glypican 3; glypican3; glypican-3; Intestinal protein OCI-5; GTR2-2; MXR7.Intestinal protein OCI-5.
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg 1ml/1mg
纯化的抗体可通过不同的途径获取,有些磷脂酰基醇蛋白聚糖-3抗体可通过下述方法制备或从商家购买。从商家购买的抗体,通常附有正确的储存方法。
1)工作液应在4℃下融化并存放,可能稳定达数月。
2)如果没有特殊原因而避免使用叠氮钠,亦可加入叠氮钠,浓度为0.02%。将纯化的抗体样本分装成合适的体积,于-20℃保存。
3)纯化的抗体溶液应以较高的浓度(如lmg/m1)在中性pH下保存。:常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法),皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记,可将它们以较低浓度储存于加有1%BSA的溶液中。
4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对磷脂酰基醇蛋白聚糖-3抗体能有损害。如果没有其他说明.律议用PBS或50mmol/LTris(DH8.0)溶液长期存放抗体。
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat
产品类型 一抗
研究领域 肿瘤 细胞生物 免疫学
蛋白分子量 predicted molecular weight: 65kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human glypican 3 C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷脂酰基醇蛋白聚糖-3抗体产品介绍 Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome. (Also known as glypican 3; Intestinal protein OCI-5; GTR2-2; MXR7; Glypican-3 precursor).
Subcellular Location : Cell membrane and Secreted > extracellular space.
Tissue Specificity : Highly expressed in lung, liver and kidney.
GPC-3磷脂酰基醇蛋白聚糖-3是一种硫酸类肝素蛋白多糖,GPC-3在不同的组织中具有不同的生物学功能,甚至起着完全不同的作用,可能与肿瘤组织类型、发病机制等密切相关.GPC3在绝大多数HCC(肝细胞癌)患者有表达。
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