G氨基丁酸受体γ1/GABAA Rγ1抗体

G氨基丁酸受体γ1/GABAA Rγ1抗体英文名称  Anti-GABRG1/GABA A Receptor gamma 1 

中文名称  G氨基丁酸受体γ1/GABAA Rγ1抗体 

别    名  GABA(A) receptor subunit gamma 1; GabaA/BZ; GABRG 1; GABRG1; Gamma aminobutyric acid (GABA A) receptor subunit gamma 1; Gamma aminobutyric acid A receptor, gamma 1; GBRG1;  

浓    度  1mg/1ml 

规 格  0.2ml/200μg     

纯化的抗体可通过不同的途径获取，有些G氨基丁酸受体γ1/GABAA Rγ1抗体可通过下述方法制备或从商家购买。从商家购买的抗体，通常附有正确的储存方法。

1）工作液应在4℃下融化并存放，可能稳定达数月。

2）如果没有特殊原因而避免使用叠氮钠，亦可加入叠氮钠，浓度为0．02％。将纯化的抗体样本分装成合适的体积，于-20℃保存。

3）纯化的抗体溶液应以较高的浓度(如lmg／m1)在中性pH下保存。：常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法)，皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记，可将它们以较低浓度储存于加有1％BSA的溶液中。

4）经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间，即使保存多年，对抗体也无损害。多数情况下，盐浓度适于保持在0-150mmol/L之间，但在长期存放的抗体中，盐溶液浓度高达500mmol/L时，对G氨基丁酸受体γ1/GABAA Rγ1抗体能有损害。如果没有其他说明．律议用PBS或50mmol/LTris(DH8．0)溶液长期存放抗体。  

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep

产品类型  一抗    

研究领域  神经生物学 细胞膜受体 G蛋白偶联受体 G蛋白信号  

蛋白分子量  predicted molecular weight: 50kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human GABRG1/GABA A Receptor gamma 1 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

G氨基丁酸受体γ1/GABAA Rγ1抗体产品介绍 GAD-65 and GAD-67, glutamate decarboxylases, function to catalyze the production of GABA (gamma-aminobutyric acid). In the central nervous system, GABA functions as the main inhibitory transmitter by increasing a Cl- (chloride) conductance that inhibits neuronal firing. GABA has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors, as well as a third class of receptors called GABAC. The Gamma subunit of GABAA receptors are important for benzodiazepine binding and modulation of GABA-mediated Cl- current. GABAA R Gamma 1 (gamma-aminobutyric acid (GABA) A receptor, gamma 1), also known as GABRG1, is a 465 amino acid multi-pass membrane protein belonging to the ligand-gated ionic channel (TC 1.A.9) family. GABAA R gamma 1 participates in neurotransmission inhibition and has been linked to alcohol dependence.

Function : GABA A Receptor gamma 1 belongs to the ligand gated ionic channel family. It is an integral membrane protein and plays an important role in inhibiting neurotransmission by binding to the benzodiazepine receptor and opening an integral chloride channel.

Subunit : Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

Subcellular Location : Cell junction, synapse, postsynaptic cell membrane; Multi pass membrane protein. 

Post-translational modifications : May be palmitoylated

Similarity : Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG1 sub-subfamily.

Database links : UniProtKB/Swiss-Prot: Q8N1C3.2