甘氨酸受体α3/GlyR α3抗体

甘氨酸受体α3/GlyR α3抗体英文名称  Anti-GLRA3/Glycine Receptor alpha 3 

中文名称  甘氨酸受体α3/GlyR α3抗体  

别    名  GLRA 3; Glra3; GLRA3_HUMAN; Glycine receptor alpha 3 polypeptide; Glycine receptor alpha 3 subunit; Glycine receptor subunit alpha 3; Glycine receptor subunit alpha-3; Ligand gated ion channel. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg     

纯化的抗体可通过不同的途径获取，有些甘氨酸受体α3/GlyR α3抗体可通过下述方法制备或从商家购买。从商家购买的抗体，通常附有正确的储存方法。

1）工作液应在4℃下融化并存放，可能稳定达数月。

2）如果没有特殊原因而避免使用叠氮钠，亦可加入叠氮钠，浓度为0．02％。将纯化的抗体样本分装成合适的体积，于-20℃保存。

3）纯化的抗体溶液应以较高的浓度(如lmg／m1)在中性pH下保存。：常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法)，皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记，可将它们以较低浓度储存于加有1％BSA的溶液中。

4）经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间，即使保存多年，对抗体也无损害。多数情况下，盐浓度适于保持在0-150mmol/L之间，但在长期存放的抗体中，盐溶液浓度高达500mmol/L时，对甘氨酸受体α3/GlyR α3抗体能有损害。如果没有其他说明．律议用PBS或50mmol/LTris(DH8．0)溶液长期存放抗体。  

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Rabbit

产品类型  一抗    

研究领域  神经生物学 通道蛋白 细胞膜受体 G蛋白偶联受体 G蛋白信号  

蛋白分子量  predicted molecular weight: 50kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human GLRA3/Glycine Receptor alpha 3 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

甘氨酸受体α3/GlyR α3抗体产品介绍 In the central nervous system (CNS), glycine-mediated inhibitory neurotransmission is essential to voluntary motor control and reflex responses. Glycine binds to glycine receptors (GlyR) in the post synaptic neuronal membranes. GlyR, Gamma-aminobutryic acid, serotonin and acetylcholine comprise an evolutionally conserved superfamily of ligand-gated ion channels. The pentameric subunit structure of GlyR consists of two types of glycosylated membrane proteins, alpha 1 through alpha 4 and Beta, and an associated peripheral membrane protein, which combine to form a chloride-selective ion channel. In humans, the composition of the pentamer changes from alpha 2 subunits in the fetal CNS to alpha 1 and Beta subunits in the adult CNS. Fast potentiation of GlyR by intracellular Ca2+ in the brainstem and midbrain indicate an important role for Ca2+ in modulation glycinergic synapses. The genes encoding human GlyR alpha 1, alpha 2, alpha 3 and Beta subunits map to chromosomes 5q33.1, Xp22.2, 4q34.1 and 4q32.1, respectively.

Function : The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).

Subunit : Pentamer composed of alpha and beta subunits

Subcellular Location : Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Tissue Specificity : Widely distributed throughout the central nervous system.

Similarity : Belongs to the ligand-gated ion channel (TC 1.A.9) family.

Glycine receptor (TC 1.A.9.3) subfamily. GLRA3 sub-subfamily.

Database links : UniProtKB/Swiss-Prot: O75311.2