磷酸化乙酰辅酶A羧化酶1ACCα抗体

英文名称  Anti-Phospho-Acetyl CoA Carboxylase(Ser79)/ACACA 

中文名称  磷酸化乙酰辅酶A羧化酶1ACCα抗体 

别    名  p-ACACA(Phospho-Ser79); ACAC; ACACA; ACACA; ACACA_HUMAN; ACC alpha; ACC-alpha; ACC1; ACC1; ACCA; Acetyl Coenzyme A; Biotin carboxylase; ACACA_MOUSE; ACACA_RAT. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg   

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, Guinea Pig   

产品类型  一抗  磷酸化抗体   

研究领域  肿瘤 细胞生物 信号转导 转录调节因子 激酶和磷酸酶 新陈代谢  

蛋白分子量  predicted molecular weight: 266kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthesised phosphopeptide derived from human Acetyl CoA Carboxylase around the phosphorylation site of Ser79 

磷酸化乙酰辅酶A羧化酶1ACCα抗体亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

磷酸化乙酰辅酶A羧化酶1ACCα抗体产品介绍 Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl-CoA carboxylase in human muscle. ACC alpha (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACC∫ (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACC alpha is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the enzyme’s short-term regulatory mechanism. The gene encoding ACC alpha maps to human chromosome 17 and encodes a form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACC∫ is homologous to that of the ACCå, except for an additional peptide of about 150 amino acids at the N-terminus.

Function : Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. 

Subunit : Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity. 

Subcellular Location : Cytoplasm.

Tissue Specificity : Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. 

Post-translational modifications : Phosphorylation on Ser-1263 is required for interaction with BRCA1. 

DISEASE : Defects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth. 

Similarity : Contains 1 ATP-grasp domain.

Contains 1 biotin carboxylation domain.

Contains 1 biotinyl-binding domain.

Contains 1 carboxyltransferase domain.