ABI1/SSH3BP1蛋白抗体

英文名称  Anti-ABI1 

中文名称  ABI1/SSH3BP1蛋白抗体 

别    名  Abelson interactor 1; Abelson interactor 1; ABI 1; ABI1; ABI-1 Abl binding protein 4; Abl interactor 1; Abl interactor protein 1 long; AblBP4; Ablphilin 1; E3B1; Eps8 binding protein; Eps8 SH3 domain binding protein; Hssh3bp1; NAP1; NAP1BP; Spectrin SH3 domain binding protein 1; SSH3BP; SSH3BP1; ABI1_HUMAN. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg  

抗体来源  Rabbit  

克隆类型  polyclonal 

ABI1/SSH3BP1蛋白抗体交叉反应  Human, Mouse, Rat, Chicken, Dog, Horse, Rabbit  

产品类型  一抗    

研究领域  免疫学 信号转导 转录调节因子  

蛋白分子量  predicted molecular weight: 55kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human ABI1 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

ABI1/SSH3BP1蛋白抗体产品介绍 May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. In vitro, at least isoform 2 and isoform 4 suppress the transforming activity of Abelson murine leukemia virus(v-Abl) after overexpression in fibroblasts. May play a role in regulation EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA (By similarity). Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level.

Function : May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. In vitro, at least isoform 2 and isoform 4 suppress the transforming activity of Abelson murine leukemia virus(v-Abl) after overexpression in fibroblasts. May play a role in regulation EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA (By similarity). Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level.

Subunit : Interacts with ABL1, ENAH, STX1A, SNAP25, VAMP2, EPS8, and through its N-terminus with WASF1. Part of a complex consisting of ABI1, STX1A and SNAP25. Part of a complex consisting of ABI1, EPS8 and SOS1 (By similarity). Interacts with SOS1, SOS2, GRB2, SPTA1 and the first SH3 domain of NCK1. Isoform 6 does not interact with NCK1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2.

Subcellular Location : Cytoplasm (By similarity). Nucleus (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, filopodium (By similarity). Cell projection, growth cone (By similarity). Cell junction, synapse, synaptosome (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes (By similarity).

Tissue Specificity : Widely expressed, with highest expression in brain.

Post-translational modifications : In vitro substrate for v-Abl. Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl (By similarity).

Similarity : Belongs to the ABI family. Contains 1 SH3 domain. Contains 1 t-SNARE coiled-coil homology domain.

Database links : UniProtKB/Swiss-Prot: Q8IZP0.4