丝氨酸抑制蛋白激酶C底物抗体

英文名称  Anti-AKAP12/Gravin 

中文名称  丝氨酸抑制蛋白激酶C底物抗体 

别    名  A kinase (PRKA) anchor protein (gravin) 12; A kinase Anchor Protein 12; A kinase anchor protein 250kDa; AKAP 12; AKAP 250; AKAP250; DKFZp686M0430; DKFZp686O0331; Gercelin; Gravin; Kinase scaffold protein gravin; Myasthenia gravis autoantigen gravin; Srcs5; SSeCKS; Tsga12. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg    

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog  

产品类型  一抗    

丝氨酸抑制蛋白激酶C底物抗体研究领域  神经生物学 信号转导 激酶和磷酸酶  

蛋白分子量  predicted molecular weight: 185kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from the middle of human AKAP12 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

丝氨酸抑制蛋白激酶C底物抗体产品介绍 Gravin (also known as A Kinase Anchor Protein 12, AKAP12) was cloned from a cDNA expression library screened with serum, from a myasthenia gravis patient. Antibodies to Gravin were found in 22 out of 72 myasthemia gravis patients and absent in sera of normal individuals. This protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain. Gravin can bind kinases like PKA and PKC by different domains of the protein and can localize these kinases to specific domains in the cell. Upon differentiation of NT2 cells to neurons (NT2 N), Gravin expression is up regulated and enriched at the inner peripheral cortex in close proximity to the plasma membrane. In differentiated cells, gravin is co-localized with PKCbII, PKCa and PKA. Decreased binding of PKCa to gravin was found after activation of PKC.

Function : Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).

Subcellular Location : Cytoplasm

Tissue Specificity : Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity : Contains 3 AKAP domains.