抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Arabidopsis Thaliana
产品类型 一抗 植物抗体
研究领域 细胞生物 免疫学 转录调节因子 植物
蛋白分子量 predicted molecular weight: 104kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from Arabidopsis thaliana (thale cress) AHA3 C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
AHA3抗体(拟南芥)产品介绍
Function : The plasma membrane H(+) ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
Subunit : Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-948. Binding to 14-3-3 proteins activates the H(+)-ATPase.
Subcellular Location : Cell membrane; Multi-pass membrane proteinc(Probable).
Tissue Specificity : Found predominantly in phloem cells of leaves, stems, roots and flowers.
Post-translational modifications : Phosphorylation level varies significantly during early response to bacterial elicitor (e.g. fusicoccin FC).
Similarity : Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.
Database links : UniProtKB/Swiss-Prot: P20431.2
纯度:在实验的任何阶段,确定抗体溶液纯度的最简单方法是取一部分样本进行SDS-PAGE电泳。凝胶可用考马斯亮蓝染色(灵敏度为0.1—0.5ug/带)或银染(灵敏度1~l0ug/带)。
定量:如果抗体还不纯,有一个快捷的定量方法,即通过SDS-PAGE电泳分离出轻、重链,然后和已知的标准染色带比较。如果需要分析许多样本,用免疫测定法对抗体定量较容易。如果抗体是经过纯化的,可通过测蛋白总量代替上述两种方法,有一简单的方法,即紫外吸收法。AHA3抗体(拟南芥)的量可通过测280nm处的吸收值来测(10D大致相当于0.75mg/m1的纯化抗体)。
抗原结合活性:一般说来,纯化方法不会引起抗原结合活性的改变。用蛋白G或蛋白A树脂很少导致抗体活性丧失。然而,如果最终抗体产物的作用不如原来所预料的好,检测抗体纯化过程所丢失的活性就极为重要。用一系列滴定法比较纯化的抗体和其原材料的活性,以标定每一步中的总抗体量,这将有助于较好的估计通过纯化所丢失的活性。