磷酸化非受体性蛋白酪氨酸激酶ETK抗体

磷酸化非受体性蛋白酪氨酸激酶ETK抗体英文名称  Anti-phospho-BMX (Tyr566) 

中文名称  磷酸化非受体性蛋白酪氨酸激酶ETK抗体 

别    名  BMX (phospho Y556); BMX (phospho Try556); BMX (p-Y556); BMX (p-Try556); p-BMX (phospho Y556); Protein tyrosine kinase BMX; PSCTK 2; PSCTK 3; PSCTK2; PSCTK3; Bone marrow tyrosine kinase gene in chromosome X protein; BMX non receptor tyrosine kinase; Cytoplasmic tyrosine protein kinase BMX; Epithelial and endothelial tyrosine kinase; BMX_HUMAN; Cytoplasmic tyrosine-protein kinase BMX; ETK; NTK38; Protein tyrosine kinase BMX; Etk; Etk/Bmx; Tyro8. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg      

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep  

产品类型  一抗  磷酸化抗体   

研究领域  细胞生物 信号转导 激酶和磷酸酶  

蛋白分子量  predicted molecular weight: 78kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human ETK around the phosphorylation site of Tyr566 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

磷酸化非受体性蛋白酪氨酸激酶ETK抗体产品介绍 Tyrosine kinases are either receptor molecules, which contain transmembrane and extracellular domains, or nonreceptor proteins, which are located intracellularly. BMX[ETK] is a member of Bruton's tyrosine kinase family. It is expressed in haematopoetic, epithelial and endothelial cells and participates in multiple signal transduction pathways.

Function : Non-receptor tyrosine kinase that plays central butdiverse modulatory roles in various signaling processes involved inthe regulation of actin reorganization, cell migration, cellproliferation and survival, cell adhesion, and apoptosis.Participates in signal transduction stimulated by growth factorreceptors, cytokine receptors, G-protein coupled receptors, antigenreceptors and integrins. Induces tyrosine phosphorylation of BCAR1in response to integrin regulation. Activation of BMX by integrinsis mediated by PTK2/FAK1, a key mediator of integrin signalingevents leading to the regulation of actin cytoskeleton and cellmotility. Plays a critical role in TNF-induced angiogenesis, andimplicated in the signaling of TEK and FLT1 receptors, 2 importantreceptor families essential for angiogenesis. Required for thephosphorylation and activation of STAT3, a transcription factorinvolved in cell differentiation. Also involved in interleukin-6(IL6) induced differentiation. Plays also a role in programmingadaptive cytoprotection against extracellular stress in differentcell systems, salivary epithelial cells, brain endothelial cells,and dermal fibroblasts. May be involved in regulation ofendocytosis through its interaction with an endosomal proteinRUFY1. May also play a role in the growth and differentiation ofhematopoietic cells; as well as in signal transduction inendocardial and arterial endothelial cells.as in signal transduction inendocardial and arterial endothelial cells.

Subunit : Interacts with BCAR1, CAV1, MYD88, PTK2/FAK1, RUFY1,RUFY2, STAT3, TIRAP and TNFRSF1B.

Subcellular Location : Cytoplasm. Note=Localizes to the edges ofspreading cells when complexed with BCAR1.

Tissue Specificity : Highly expressed in cells with great migratorypotential, including endothelial cells and metastatic carcinomacell lines.

Post-translational modifications : Phosphorylated in response to protein I/II and to LPS.Phosphorylation at Tyr-566 by SRC and by autocatalysis leads toactivation and is required for STAT3 phosphorylation by BMX.

Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family.

TEC subfamily.

Contains 1 Btk-type zinc finger. Contains 1 PH domain.

Contains 1 protein kinase domain.

Contains 1 SH2 domain.

Database links : UniProtKB/Swiss-Prot: P51813