STAT3抗体说明书

Rabbit Anti-STAT3抗体说明书antibody

Catalog Number : ab68153

Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ） Background: background:
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. Gene Symbol: APRF; FLJ20882; HIES; MGC16063.

Specificity :

–Anti-STAT3 is a rabbit monoclonal antibody

–specific for STAT3 of Mouse, Rat, Human

– use for western blotting,elisa,immunoprecipitation and immunohistochemistry

–Protein G affinity chromatography purification, purity :>95%

– Isotype: IgG

– mol wt: 88 kDa

Application :

WB=1:100-500

IHC-P=1:100-500

IHC-F=1:100-500

– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 STAT3抗体说明书相关标记：FITC、Gold 、HRP、PE   PE-Cy3、PE-CY5、PE-CY5.5 、PE-CY7 、RBITC 、  Alexa Fluor 350、Alexa Fluor 488 、 Alexa Fluor 555 、Alexa Fluor 647、AP 、APC  、Biotin 、Cy3 、Cy5 、Cy5.5 、Cy7 、
浓    度：  1mg/1ml
抗体来源（宿主）：兔源、鼠源、其他 
克隆：单克隆抗体、多克隆抗体
适用：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, Monkey,  others。
抗体类型：一抗   
研究领域:细胞生物、神经生物学等 
性    状：  冻干粉或液体
储 存 液： Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 or PBS with 0.1% sodium azide and 50% glycerol pH 7.3. -20oC, Avoid freeze / thaw cycles.
产品应用    Immunohistochemistry （IHC）, Flow Cytometry （FACS） , Western Blotting （WB） , ELISA , Immunohistochemistry , Immunohistochemistry （Paraffin-embedded Sections） （IHC （p）） , Immunoprecipitation （IP） , Immunocytochemistry （ICC） ，Immunofluorescence （IF）等。