CD3抗体，CD3抗体说明书

上海瑞齐生物技术有限公司

2014/06/26 07:59

Anti- CD3/CD3抗体说明书

From:ABCAM

Catalog Number :RS- 0765R

Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent ) Background: The human CD3/T cell receptor (TcR) complex is made up of at least five CD3 proteins (gamma, delta, epsilon, h, z) in association with either alpha/beta or gamma/delta proteins of the TcR (2). The TcR recognizes antigens in association with MHC molecules after which protein chains of the CD3 complex mediate activation signals triggered by TcR antigen binding. CD3 is expressed on greater than 95% of circulating human peripheral T cells. The CD3 complex mediates signal transduction.
Function : The CD3 complex mediates signal transduction.
Subunit : The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.
Subcellular Location : Membrane; Single-pass type I membrane protein.
Similarity : Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.

.Specificity : Anti - CD3 is a mouse monoclonal antibody

specific forCD3 of Human

use for western blotting,elisa,immunoprecipitation and immunohistochemistry

Protein G affinity chromatography purification, purity :>95%

Isotype: IgG

mol wt: 20kDa

Application :

WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

KLH conjugated synthetic peptide derived from human CD3 C-terminus

not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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