CK18绿色抗体，CK18标记一抗说明书

上海瑞齐生物技术有限公司

2014/06/26 08:02

Anti- CK18绿色抗体，CK18标记一抗说明书

From:ABCAM

Catalog Number :RS- 2043R

Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent ) Background: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
Subunit : Heterotetramer of two type I and two type II keratins. KRT18 associates with KRT8. Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with the thrombin-antithrombin complex. Interacts with DNAJB6, TCHP and TRADD (By similarity).
Tissue Specificity : Expressed in endoderm, intestinal epithelial cells and in most extraembryonic tissues.
Similarity : Belongs to the intermediate filament family.

.Specificity : Anti –CK18 is a rabbit polyclonal antibody

specific for CK18 of Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,

use for western blotting,elisa,immunoprecipitation and immunohistochemistry

Protein G affinity chromatography purification, purity :>95%

Isotype: IgG

mol wt: 48kDa

Application :

WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 KLH conjugated synthetic peptide derived from human CK18 (53-96aa)

not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

CK18绿色抗体，CK18标记一抗说明书

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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