Anti-capsid protein抗体

上海瑞齐生物技术有限公司

2015/07/08 08:00

mouse Anti-capsid protein抗体 antibody

Catalog Number : RS-6432R

Quantity size : 100ul (dilute with pH 7.4 0.01 M PBS or antibody diluent )

background:
There has been much uncertainty in the relationship between PCV2 and PMWS but Bolin et al demonstrated that Koch's postulates could be fulfilled by inoculating cesarean-derived, colostrum-deprived (CDCD) pigs with PCV2 and producing clinical signs of PMWS. Similar work has been performed using an infectious genomic clone of PCV2 in specific pathogen free (SPF) pigs to produce the gross and microscopic lymphoid lesions of PMWS. Although PCV2 has been shown to induce PMWS lesions in CDCD and SPF pigs, the presence of additional factors are required.

Function:

Self-assembles to form the virion icosahedral capsid with a T=1 symmetry. This very small capsid (17 - 22 nm in diameter) allows the virus to be very stable in the environment and resistant to some disinfectants, including detergents. Essential for the initial attachment to heparan sulfate moities and chondroitin sulfate B of the host cell surface proteoglycans. After attachment, the virus is internalized in a clathrin-, caveolae- and dynamin-independent, actin and Rho-GTPase-mediated pathway and traffics to the nucleus. The capsid protein binds and transports the viral genome and Rep across the nuclear envelope.

Specificity : Anti-capsid protein is a mouse monoclonal antibody

specific for anti-capsid protein of Human, Mouse, Rat, Dog, Horse, Rabbit

- use for western blotting,elisa,immunoprecipitation and immunohistochemistry

– Protein G affinity chromatography purification, purity :>95%

– Isotype: IgG

– mol wt: 26 kDa

Application :

– WB=1:100-500

– ELISA=1:500-1000

– IHC-P=1:100-500

– IHC-F=1:100-500

– optimal dilutions/concentrations should be determined by the end user.

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.Anti-capsid protein抗体

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